| pubmed-article:6935665 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C1179435 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C1705248 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C1548799 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C1524073 | lld:lifeskim |
| pubmed-article:6935665 | lifeskim:mentions | umls-concept:C0449432 | lld:lifeskim |
| pubmed-article:6935665 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:6935665 | pubmed:dateCreated | 1981-3-24 | lld:pubmed |
| pubmed-article:6935665 | pubmed:abstractText | The regulatory component (G/F) of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] from rabbit liver plasma membranes has been purified essentially to homogeneity. The purification was accomplished by three chromatographic procedures in sodium cholate-containing solutions, followed by three steps in Lubrol-containing solutions. The specific activity of G/F was enriched 2000-fold from extracts of membranes to 3-4 mumol x min-1 x mg-1 (reconstituted adenylate cyclase activity). Purified G/F reconstitutes guanine nucleotide-, fluoride-, and hormone-stimulated adenylate cyclase activity in the adenylate cyclase-deficient variant of S49 murine lymphoma cells. G/F also recouples hormonal stimulation of the enzyme in the uncoupled variant of S49. Preparations of pure G/F contain three polypeptides with approximate molecular weights of 52,000, 45,000, and 35,000. The active G/F protein behaves as a multisubunit complex of these polypeptides. Treatment of G/F with [32P]NAD+ and cholera toxin covalently labels the molecular weight 52,000 and 45,000 polypeptides with 32P. | lld:pubmed |
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| pubmed-article:6935665 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6935665 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6935665 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6935665 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6935665 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:6935665 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6935665 | pubmed:author | pubmed-author:GilmanA GAG | lld:pubmed |
| pubmed-article:6935665 | pubmed:author | pubmed-author:RossE MEM | lld:pubmed |
| pubmed-article:6935665 | pubmed:author | pubmed-author:NorthupJ KJK | lld:pubmed |
| pubmed-article:6935665 | pubmed:author | pubmed-author:SmigelM DMD | lld:pubmed |
| pubmed-article:6935665 | pubmed:author | pubmed-author:SternweisP... | lld:pubmed |
| pubmed-article:6935665 | pubmed:author | pubmed-author:SchleiferL... | lld:pubmed |
| pubmed-article:6935665 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6935665 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:6935665 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6935665 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6935665 | pubmed:pagination | 6516-20 | lld:pubmed |
| pubmed-article:6935665 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6935665 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6935665 | pubmed:articleTitle | Purification of the regulatory component of adenylate cyclase. | lld:pubmed |
| pubmed-article:6935665 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6935665 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6935665 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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