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pubmed-article:663956pubmed:abstractTextThe possibility of quantitating insect ecdysones in situ on thin-layer chromatographic plates was examined. Two approaches were evaluated: 1) the induction of ecdysone fluorescence by sulfuric acid treatments and 2) the fluorescence-quenching of fluorescent thin-layer chromatographic plates by ecdysones. The fluorescence-quenching method was found to be most suitable and had a linear response range from 0.5 to 3 microgram for alpha-ecdysone and 20-hydroxyecdysone. Fluorescence-quenching and high pressure liquid chromatographic analyses obtained from extracts of alpha-ecdysone 20-hydroxylase incubations gave similar results. New data concerning the acid-induced fluorescence of ecdysones showed alpha-ecdysone to be twice as fluorescent as 20-hydroxyecdysone.lld:pubmed
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pubmed-article:663956pubmed:authorpubmed-author:SvobodaJ AJAlld:pubmed
pubmed-article:663956pubmed:authorpubmed-author:MayerR TRTlld:pubmed
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pubmed-article:663956pubmed:volume31lld:pubmed
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pubmed-article:663956pubmed:pagination139-50lld:pubmed
pubmed-article:663956pubmed:dateRevised2003-11-14lld:pubmed
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pubmed-article:663956pubmed:year1978lld:pubmed
pubmed-article:663956pubmed:articleTitleThin-layer chromatographic in situ analysis of insect ecdysones via fluorescence-quenching.lld:pubmed
pubmed-article:663956pubmed:publicationTypeJournal Articlelld:pubmed