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pubmed-article:6533152pubmed:abstractTextQuantitative electron-probe energy dispersive X-ray microanalysis has, for the first time, been accomplished at a subcellular level in plant tissue using cryofixed and thin freeze-dried cryosections. The subcellular concentrations of Na+, Cl-, K+, P, S, Mg2+ and Ca2+ were measured in mol/kg dry weight in two types of root meristematic cells of the onion, Allium cepa. The cell wall of the meristematic cells had much higher concentrations of K+ and Ca2+ than was found in the intracellular compartments. Storage granules in the protoderm cells were about 6-12 times lower in P and were about four times higher in S as compared to other intracellular compartments. Comparison between the concentrations of ions and other elements in meristematic plant cells and in mouse cardiac myocytes confirms that major differences in cytoplasmic Na+ and Cl- concentrations do indeed exist between these cell types.lld:pubmed
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pubmed-article:6533152pubmed:articleTitleThe subcellular concentration of ions and elements in thin cryosections of onion root meristem cells. An electron-probe EDS study.lld:pubmed
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pubmed-article:6533152pubmed:publicationTypeComparative Studylld:pubmed
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