| pubmed-article:6455240 | pubmed:abstractText | Cell cultures propagated from fetal bovine ligamentum nuchae were found to accumulate an extensive extracellular matrix containing collagen and elastic fibers when maintained for periods up to 8 weeks. The synthesis by these cells of two glycoproteins, designated MFP I and MFP II, which are immunologically related to components of the elastic fiber microfibrils was investigated. MFP II, apparent mol. t. 300,000, was shown to be a non-collagenous glycoprotein whereas MFP I, apparent mol. wt. 150,000, was found to be a novel collagenous glycoprotein containing hydroxyproline and glycosylated hydroxylysine residues but exhibiting properties of distinct from the known collagens. Two glycoproteins having characteristics similar to MFP I and MFP II were also identified in an extract of the cell layer and in a "microfibrillar protein preparation" from intact nuchal ligament. | lld:pubmed |
