| pubmed-article:641031 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:641031 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:641031 | lifeskim:mentions | umls-concept:C1149851 | lld:lifeskim |
| pubmed-article:641031 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:641031 | lifeskim:mentions | umls-concept:C0055571 | lld:lifeskim |
| pubmed-article:641031 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:641031 | pubmed:dateCreated | 1978-6-28 | lld:pubmed |
| pubmed-article:641031 | pubmed:abstractText | A new enzymatic method was developed for the assay of phospholipase D [phosphatidylcholine phosphatidohydrolase EC 3.1.4.4] from cabbage leaves using choline oxidase from Arthrobacter globiformis cells. The method was based on the estimation of choline by the following series of enzymatic reactions after ending the phospholipase D reaction: Choline + 202 + h2o Choline oxidase Betaine + 2H2O2 2H2O2 + Phenol + 4-Aminoantipyrine Peroxidase Quinoneimine dye + 4H2O The amount of choline was proportional to the amount of resulting quinoneimine dye with an absorbance maximum at 500 nm. The phospholipase D reaction (choline liberation) was carried out at pH 5.5 in the presence of Ca2+ ions and ended by adding EDTA in conc. Tris-HCl buffer, pH 8, to give a final pH of around 8. The initial rate of the phospholipase D reaction was proportional to the enzyme concentration over the absorbance change range of 0 to 0.25 (equivalent to 0-21 micron of choline) under the optimal reaction conditions. | lld:pubmed |
| pubmed-article:641031 | pubmed:language | eng | lld:pubmed |
| pubmed-article:641031 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:641031 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:641031 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:641031 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:641031 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:641031 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:641031 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:641031 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:641031 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:641031 | pubmed:issn | 0021-924X | lld:pubmed |
| pubmed-article:641031 | pubmed:author | pubmed-author:ImamuraSS | lld:pubmed |
| pubmed-article:641031 | pubmed:author | pubmed-author:HoriutiYY | lld:pubmed |
| pubmed-article:641031 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:641031 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:641031 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:641031 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:641031 | pubmed:pagination | 677-80 | lld:pubmed |
| pubmed-article:641031 | pubmed:dateRevised | 2007-12-19 | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-C... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-P... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-B... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-B... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-M... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-A... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-A... | lld:pubmed |
| pubmed-article:641031 | pubmed:meshHeading | pubmed-meshheading:641031-A... | lld:pubmed |
| pubmed-article:641031 | pubmed:year | 1978 | lld:pubmed |
| pubmed-article:641031 | pubmed:articleTitle | Enzymatic determination of phospholipase D activity with choline oxidase. | lld:pubmed |
| pubmed-article:641031 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:641031 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:641031 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:641031 | lld:pubmed |
