| pubmed-article:6324131 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C1330957 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0037874 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0040087 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0917792 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0596311 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C1709915 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0150312 | lld:lifeskim |
| pubmed-article:6324131 | lifeskim:mentions | umls-concept:C0684192 | lld:lifeskim |
| pubmed-article:6324131 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:6324131 | pubmed:dateCreated | 1984-5-11 | lld:pubmed |
| pubmed-article:6324131 | pubmed:abstractText | A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal lamp emitting mainly 254-nm light in the presence of 1 M spermine in distilled water resulted in a T specific cleavage of the DNA chains. This method does not require piperidine treatment. By contrast, when the DNA fragments were irradiated in the presence of methylamine under similar conditions, both G and T bands with the intensity of G greater than T have appeared. A similar but less selective T cleavage has also been observed in the irradiation of 5'-[32P]-end-labeled DNA fragments in the presence of spermine followed by brief heating of the photolysate in a loading buffer for gel electrophoresis. The T specific photoreaction with spermine and the G greater than T reaction with methylamine described here may be conveniently used in combination with the standard Maxam-Gilbert's reactions to provide independent confirmatory readings. | lld:pubmed |
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| pubmed-article:6324131 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6324131 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6324131 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6324131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6324131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:6324131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6324131 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6324131 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6324131 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:6324131 | pubmed:author | pubmed-author:MatsuuraTT | lld:pubmed |
| pubmed-article:6324131 | pubmed:author | pubmed-author:UedaKK | lld:pubmed |
| pubmed-article:6324131 | pubmed:author | pubmed-author:SaitoII | lld:pubmed |
| pubmed-article:6324131 | pubmed:author | pubmed-author:KomanoTT | lld:pubmed |
| pubmed-article:6324131 | pubmed:author | pubmed-author:SugiyamaHH | lld:pubmed |
| pubmed-article:6324131 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6324131 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:6324131 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:6324131 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6324131 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6324131 | pubmed:pagination | 2879-85 | lld:pubmed |
| pubmed-article:6324131 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6324131 | pubmed:meshHeading | pubmed-meshheading:6324131-... | lld:pubmed |
| pubmed-article:6324131 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6324131 | pubmed:articleTitle | A new procedure for determining thymine residues in DNA sequencing. Photoinduced cleavage of DNA fragments in the presence of spermine. | lld:pubmed |
| pubmed-article:6324131 | pubmed:publicationType | Journal Article | lld:pubmed |
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