| pubmed-article:629973 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:629973 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:629973 | lifeskim:mentions | umls-concept:C0018321 | lld:lifeskim |
| pubmed-article:629973 | lifeskim:mentions | umls-concept:C0025831 | lld:lifeskim |
| pubmed-article:629973 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:629973 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:629973 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:629973 | pubmed:dateCreated | 1978-5-24 | lld:pubmed |
| pubmed-article:629973 | pubmed:abstractText | tRNA(guanine-1-)-methyltransferase (EC 2.1.1.31) and tRNA(N2-guanine)-methyltransferase I (EC 2.1.1.32) were isolated from rat liver. The (guanine-1-)-methyltransferase preparation is 6800-fold purified and is free from contaminating methyltransferases or ribonuclease. The molecular weight of (guanine-1-)-methyltransferase is 83 000. Of seven purified Escherichia coli tRNAs examined, only tRNAMetf was utilized as substrate by (guanine-1-)-methyltransferase. The methylation of tRNAMetf is maximally stimulated by 40 mM putrescine with a pH optimum of 8.0. Using E. coli K-12 tRNA, the Km for S-adenosylmethionine is 3 micrometer and Ki for S-adenosylhomocysteine is 0.11 micrometer for (guanine-1-)-methyltransferase. (N2-Guanine-)-methyltransferase is 6200-fold purified and is also free of interfering enzymes. It has a molecular weight of 69 000. E. coli tRNAPhe, tRNAVal and tRNAArg are substrates for this enzyme which introduces a methyl at the 2-amino group of the guanine at position 10 from the 5'-terminus of these tRNAs. The methylation of tRNAPhe is maximally stimulated by 100 micrometer spermidine with a pH optimum of 8.0. (N2-Guanine-)-methyltransferase has a Km for S-adenosylmethionine of 2 micrometer and a Ki for S-adenosylhomocysteine of 23 micrometer with E. coli K-12 tRNA as methyl acceptor. | lld:pubmed |
| pubmed-article:629973 | pubmed:language | eng | lld:pubmed |
| pubmed-article:629973 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:629973 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:629973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:629973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:629973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:629973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:629973 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:629973 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:629973 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:629973 | pubmed:author | pubmed-author:LeboyP SPS | lld:pubmed |
| pubmed-article:629973 | pubmed:author | pubmed-author:GlickJ MJM | lld:pubmed |
| pubmed-article:629973 | pubmed:author | pubmed-author:AveryhartV... | lld:pubmed |
| pubmed-article:629973 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:629973 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:629973 | pubmed:volume | 518 | lld:pubmed |
| pubmed-article:629973 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:629973 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:629973 | pubmed:pagination | 158-71 | lld:pubmed |
| pubmed-article:629973 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:629973 | pubmed:meshHeading | pubmed-meshheading:629973-A... | lld:pubmed |
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| pubmed-article:629973 | pubmed:meshHeading | pubmed-meshheading:629973-K... | lld:pubmed |
| pubmed-article:629973 | pubmed:meshHeading | pubmed-meshheading:629973-P... | lld:pubmed |
| pubmed-article:629973 | pubmed:meshHeading | pubmed-meshheading:629973-S... | lld:pubmed |
| pubmed-article:629973 | pubmed:meshHeading | pubmed-meshheading:629973-S... | lld:pubmed |
| pubmed-article:629973 | pubmed:meshHeading | pubmed-meshheading:629973-t... | lld:pubmed |
| pubmed-article:629973 | pubmed:year | 1978 | lld:pubmed |
| pubmed-article:629973 | pubmed:articleTitle | Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver. | lld:pubmed |
| pubmed-article:629973 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:629973 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:629973 | lld:pubmed |
