Linked Life Data

6296041

Source:http://linkedlifedata.com/resource/pubmed/id/6296041

Statements in which the resource exists.
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pubmed-article:6296041pubmed:abstractTextA technique was developed that permits the analysis of large regions of DNA by transposition mutagenesis. Large fragments of the pTiA6NC plasmid were cloned into the broad host range cosmid pHK17 and subjected to transposition mutagenesis by Tn3. Cosmids containing Tn3 insertions were selected by in vivo packaging by lambda cI857 and transduction to a new host. The insertions were localized by DNA restriction endonuclease analysis and transferred to the Ti-plasmid by marker exchange.lld:pubmed
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pubmed-article:6296041pubmed:articleTitleIn vivo packaging of cosmids in transposon-mediated mutagenesis.lld:pubmed
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