pubmed-article:6271645 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C0038420 | lld:lifeskim |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C0030176 | lld:lifeskim |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C0443288 | lld:lifeskim |
pubmed-article:6271645 | lifeskim:mentions | umls-concept:C1706536 | lld:lifeskim |
pubmed-article:6271645 | pubmed:issue | 2-3 | lld:pubmed |
pubmed-article:6271645 | pubmed:dateCreated | 1982-1-9 | lld:pubmed |
pubmed-article:6271645 | pubmed:abstractText | Deletion mutants of the temperate Streptomyces phage phi C31 were selected by two methods: resistance to the chelating agent sodium pyrophosphate, and plating of a phi C31::pBR322 hybrid phage on Streptomyces albus G to obtain large plaque mutants. The deletions defined a 7.7 kilobase (kb) segment of the phi C31 genome which is inessential for plaque formation, in addition to a shorter segment including the repressor gene. Analysis of deletions and insertions suggested that the minimum size of the phi C31 genome allowing plaque formation is 37.5 kb (91% of the wild-type length of 41.2 kb), and the maximum is at least 42.4 kb (103%). These results indicate that it should be possible to introduce up to 10 kb of foreign DNA into a suitably developed phi C31 vector. | lld:pubmed |
pubmed-article:6271645 | pubmed:language | eng | lld:pubmed |
pubmed-article:6271645 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6271645 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:6271645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6271645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6271645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6271645 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:6271645 | pubmed:month | Nov | lld:pubmed |
pubmed-article:6271645 | pubmed:issn | 0378-1119 | lld:pubmed |
pubmed-article:6271645 | pubmed:author | pubmed-author:BrutonC JCJ | lld:pubmed |
pubmed-article:6271645 | pubmed:author | pubmed-author:SpringerWW | lld:pubmed |
pubmed-article:6271645 | pubmed:author | pubmed-author:SuarezJ EJE | lld:pubmed |
pubmed-article:6271645 | pubmed:author | pubmed-author:ChaterK FKF | lld:pubmed |
pubmed-article:6271645 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:6271645 | pubmed:volume | 15 | lld:pubmed |
pubmed-article:6271645 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:6271645 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:6271645 | pubmed:pagination | 249-56 | lld:pubmed |
pubmed-article:6271645 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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pubmed-article:6271645 | pubmed:meshHeading | pubmed-meshheading:6271645-... | lld:pubmed |
pubmed-article:6271645 | pubmed:year | 1981 | lld:pubmed |
pubmed-article:6271645 | pubmed:articleTitle | Dispensable sequences and packaging constraints of DNA from the Streptomyces temperate phage phi C31. | lld:pubmed |
pubmed-article:6271645 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:6271645 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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