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pubmed-article:6264047pubmed:abstractTextThe sodium channel saxitoxin binding component from rat sarcolemma was solubilized with NP-40 and centrifuged on sucrose gradients constructed in either D2O or H2O. When compared with a series of standard proteins the sedimentation behavior of the solubilized channel complex changed from an apparent S20,w of 9.1 in H2O to 6.1 in D2O. From these observations, a true partial specific volume of 0.83 ml/g was calculated for the complex. A Stokes radius of 8.6 nm was estimated from Sepharose 6-B chromatography in NP-40. The calculated protein molecular weight of the lipid-protein-detergent complex based on these data is 560,000. The complex contains about 56% protein, and the calculated molecular weight of this component is 314,000 if a v for the protein of 0.74 ml/g is assumed.lld:pubmed
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pubmed-article:6264047pubmed:pagination2097-100lld:pubmed
pubmed-article:6264047pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:6264047pubmed:articleTitleEstimate of the molecular weight of the sarcolemmal sodium channel using H2O-D2O centrifugation.lld:pubmed
pubmed-article:6264047pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:6264047pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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