pubmed-article:6232897 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0016871 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0039185 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0043454 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0149313 | lld:lifeskim |
pubmed-article:6232897 | lifeskim:mentions | umls-concept:C0057445 | lld:lifeskim |
pubmed-article:6232897 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:6232897 | pubmed:dateCreated | 1984-6-20 | lld:pubmed |
pubmed-article:6232897 | pubmed:abstractText | Three methods for detecting toxigenic fusaria in culture were compared by using known producers of zearalenone, zearalenol, T-2 toxin, and deoxynivalenol. Moist, autoclaved rice cultures of known toxigenic isolates grown in 20-ml tubes yielded oily extracts containing compounds which interfered with qualitative and quantitative analysis for the mycotoxins. Vermiculite moistened with nutrient broth in 20-ml tubes yielded a much cleaner extract. Growing the fungi on a liquid medium required a shorter incubation period, but yields of T-2 toxin and deoxynivalenol were low and variable, and the method required greater space in the incubator. Screening of the extracts by thin-layer chromatography with colorimetric spray reagents to detect the presence of these toxins permitted reduction in the number of extracts quantified by the more lengthy gas-liquid chromatographic method. Culturing in nutrient broth on vermiculite in tubes coupled to a qualitative screen before quantitation proved to be a convenient, inexpensive, and relatively rapid method that enabled reliable screening of a large number of Fusarium isolates for toxin production as compared with prior methods. | lld:pubmed |
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pubmed-article:6232897 | pubmed:language | eng | lld:pubmed |
pubmed-article:6232897 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6232897 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:6232897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:6232897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:6232897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6232897 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:6232897 | pubmed:month | Apr | lld:pubmed |
pubmed-article:6232897 | pubmed:issn | 0099-2240 | lld:pubmed |
pubmed-article:6232897 | pubmed:author | pubmed-author:RichardsonK... | lld:pubmed |
pubmed-article:6232897 | pubmed:author | pubmed-author:HamiltonP BPB | lld:pubmed |
pubmed-article:6232897 | pubmed:author | pubmed-author:HaglerW MWMJr | lld:pubmed |
pubmed-article:6232897 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:6232897 | pubmed:volume | 47 | lld:pubmed |
pubmed-article:6232897 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:6232897 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:6232897 | pubmed:pagination | 643-6 | lld:pubmed |
pubmed-article:6232897 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:6232897 | pubmed:year | 1984 | lld:pubmed |
pubmed-article:6232897 | pubmed:articleTitle | Method for detecting production of zearalenone, zearalenol, T-2 toxin, and deoxynivalenol by Fusarium isolates. | lld:pubmed |
pubmed-article:6232897 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:6232897 | pubmed:publicationType | Comparative Study | lld:pubmed |