| pubmed-article:6200874 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6200874 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:6200874 | lifeskim:mentions | umls-concept:C0017645 | lld:lifeskim |
| pubmed-article:6200874 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:6200874 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:6200874 | lifeskim:mentions | umls-concept:C0205374 | lld:lifeskim |
| pubmed-article:6200874 | lifeskim:mentions | umls-concept:C1504308 | lld:lifeskim |
| pubmed-article:6200874 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:6200874 | pubmed:dateCreated | 1984-5-11 | lld:pubmed |
| pubmed-article:6200874 | pubmed:abstractText | We examined the effects of the simian virus 40 enhancer sequence on transcription of cloned human alpha- and beta-globin genes shortly after their introduction into cultured mammalian cells. We find that (i) detectable transcription of the beta-globin gene but not the alpha-globin gene requires linkage to the enhancer; (ii) the enhancer increases the amount of beta-globin RNA at least 100-fold but results in only a 5- to 10-fold increase in the amount of alpha-globin RNA; (iii) plasmid replication does not increase the level of beta-globin RNA, regardless of linkage to the enhancer, but does result in an approximately equal to 50-fold increase in the level of alpha-globin RNA; (iv) the enhancer is not required for and does not increase transcription of either gene in 293 cells, an adenovirus 5-transformed human kidney cell line. We also show that an enhancer sequence is not required for activity of the normally enhancer-dependent simian virus 40 early promoter in 293 cells, indicating that these cells contain a trans-acting factor(s) that circumvents the requirement for the enhancer sequence. | lld:pubmed |
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| pubmed-article:6200874 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6200874 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6200874 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6200874 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6200874 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6200874 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6200874 | pubmed:author | pubmed-author:ManiatisTT | lld:pubmed |
| pubmed-article:6200874 | pubmed:author | pubmed-author:GreenM RMR | lld:pubmed |
| pubmed-article:6200874 | pubmed:author | pubmed-author:TreismanRR | lld:pubmed |
| pubmed-article:6200874 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6200874 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:6200874 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6200874 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6200874 | pubmed:pagination | 7428-32 | lld:pubmed |
| pubmed-article:6200874 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:6200874 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6200874 | pubmed:articleTitle | cis and trans activation of globin gene transcription in transient assays. | lld:pubmed |
| pubmed-article:6200874 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6200874 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6200874 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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