| pubmed-article:6198094 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6198094 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:6198094 | lifeskim:mentions | umls-concept:C0162735 | lld:lifeskim |
| pubmed-article:6198094 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:6198094 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:6198094 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:6198094 | lifeskim:mentions | umls-concept:C0073429 | lld:lifeskim |
| pubmed-article:6198094 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6198094 | pubmed:dateCreated | 1984-3-21 | lld:pubmed |
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| pubmed-article:6198094 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6198094 | pubmed:abstractText | We report mutations affecting several steps of CoIE1 primer formation. Primer precursor forms the hybrid with the template DNA and is cleaved by RNAase H to form the primer. Point mutations at positions -264, -265, -268, and -308 (base pairs upstream of the replication origin) reduce efficiency of hybrid formation. A suppressor mutation at -18 increases the efficiency for a mutant transcript and reduces that for a wild-type transcript. Therefore, hybrid formation probably starts after transcription passes this position. A mutation at -10 affects the site of cleavage by RNAase H. The site appears to be determined by the distance from a stem-loop structure immediately upstream. A double mutation at -186 and -188 results in formation of an RNA secondary structure that prevents use of an RNAase H-cleaved transcript as primer. | lld:pubmed |
| pubmed-article:6198094 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6198094 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6198094 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6198094 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6198094 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:6198094 | pubmed:issn | 0092-8674 | lld:pubmed |
| pubmed-article:6198094 | pubmed:author | pubmed-author:MasukataHH | lld:pubmed |
| pubmed-article:6198094 | pubmed:author | pubmed-author:TomizawaJJ | lld:pubmed |
| pubmed-article:6198094 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6198094 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:6198094 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6198094 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6198094 | pubmed:pagination | 513-22 | lld:pubmed |
| pubmed-article:6198094 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:6198094 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6198094 | pubmed:articleTitle | Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication. | lld:pubmed |
| pubmed-article:6198094 | pubmed:publicationType | Journal Article | lld:pubmed |
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