| pubmed-article:6194216 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6194216 | lifeskim:mentions | umls-concept:C0022686 | lld:lifeskim |
| pubmed-article:6194216 | lifeskim:mentions | umls-concept:C0021764 | lld:lifeskim |
| pubmed-article:6194216 | lifeskim:mentions | umls-concept:C1550101 | lld:lifeskim |
| pubmed-article:6194216 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:6194216 | lifeskim:mentions | umls-concept:C0750540 | lld:lifeskim |
| pubmed-article:6194216 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:6194216 | pubmed:dateCreated | 1983-11-23 | lld:pubmed |
| pubmed-article:6194216 | pubmed:abstractText | In the absence of specific antigen stimulation, nonspecific killer cells were induced by culturing C57BL/6 lymph node or spleen cells with interleukin 2-containing supernatants. These supernatants were obtained from stimulation of either rat spleen cells with concanavalin A or a variant of the T cell lymphoma, EL4 (H-2b) with phorbol myristic acetate. The ability of the EL4 supernatant to induce nonspecific killer cells was abrogated by absorption with an interleukin 2-dependent T cell line or by concanavalin A-stimulated spleen cell blasts, but not by lipopolysaccharide-stimulated spleen cell blasts or by a non-interleukin 2-producing EL4 line. Partially purified interleukin 2 from EL4 supernatants could also support nonspecific killer cell induction. The induction of cytolytic cells by interleukin 2 is sensitive to gamma-irradiation and has a D omicron of 120 rad. The nonspecific killer cells induced are likely cytotoxic T lymphocytes; the majority of the precursor and effector cells bear the Thy-1 alloantigen marker. These nonspecific killer cells killed a broad spectrum of target cells, including concanavalin A- and lipopolysaccharide-induced splenic blasts of syngeneic or allogeneic mice, a syngeneic tumor, and a cloned allogeneic cytotoxic T cell line. The frequency of precursors for nonspecific killer cells in C57BL/6 lymph node and spleen cells are 1/7000 and 1/12,000, respectively. Clonal analyses revealed that these nonspecific killers exhibit heterogeneity with respect to their target cell specificities. The induction of nonspecific killers by interleukin 2-containing supernatants is partially dependent on nylon wool-adherent cells; in antigen-stimulated cultures the most specific killer cells were obtained from cultures in which nylon wool-nonadherent lymph node responder cells were stimulated with nylon wool-nonadherent allogeneic splenic stimulator cells that were treated with anti-Thy-1 antibody and complement. The relevance of these findings with respect to the frequencies and fine specificities of cytotoxic T lymphocytes generated in interleukin 2-supplemented cultures is discussed. | lld:pubmed |
| pubmed-article:6194216 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6194216 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6194216 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:6194216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6194216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6194216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6194216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6194216 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6194216 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:6194216 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:6194216 | pubmed:author | pubmed-author:TehH SHS | lld:pubmed |
| pubmed-article:6194216 | pubmed:author | pubmed-author:YuMM | lld:pubmed |
| pubmed-article:6194216 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6194216 | pubmed:volume | 131 | lld:pubmed |
| pubmed-article:6194216 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6194216 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6194216 | pubmed:pagination | 1827-33 | lld:pubmed |
| pubmed-article:6194216 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:meshHeading | pubmed-meshheading:6194216-... | lld:pubmed |
| pubmed-article:6194216 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6194216 | pubmed:articleTitle | Activation of nonspecific killer cells by interleukin 2-containing supernatants. | lld:pubmed |
| pubmed-article:6194216 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6194216 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6194216 | lld:pubmed |
