| pubmed-article:6187800 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6187800 | lifeskim:mentions | umls-concept:C1882810 | lld:lifeskim |
| pubmed-article:6187800 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
| pubmed-article:6187800 | lifeskim:mentions | umls-concept:C0075333 | lld:lifeskim |
| pubmed-article:6187800 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6187800 | pubmed:dateCreated | 1983-5-5 | lld:pubmed |
| pubmed-article:6187800 | pubmed:abstractText | A cytochemical procedure for the localization of adenylate cyclase with Sr2+ as the capture ion and adenylyl imidodiphosphate as the specific substrate was evaluated in the rat pancreas. Incubation medium was unaffected by the addition of 5 mM strontium ions but became turbid in the presence of lead or strontium plus 10 mM NaF. Tissues were prefixed in 2% formaldehyde/0.5% glutaraldehyde and incubated, and the cytochemical precipitate was converted to the Pb2+ salt. Enzymatic activity was demonstrated on the plasma membrane of pancreatic acinar cells and responded to stimulation by secretin. Controls frequently contained Pb2+ sequestered in mitochondria, but otherwise only a few randomly distributed grains were observed. The controls were 1) omission of substrate from the medium; 2) incubation of tissue for 1 min in complete medium; and 3) tissue previously inactivated by microwave irradiation and incubated for 30 min in complete medium including secretin. | lld:pubmed |
| pubmed-article:6187800 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6187800 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6187800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6187800 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6187800 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:6187800 | pubmed:issn | 0022-1554 | lld:pubmed |
| pubmed-article:6187800 | pubmed:author | pubmed-author:SchachtJJ | lld:pubmed |
| pubmed-article:6187800 | pubmed:author | pubmed-author:ZajicGG | lld:pubmed |
| pubmed-article:6187800 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6187800 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:6187800 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6187800 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6187800 | pubmed:pagination | 25-8 | lld:pubmed |
| pubmed-article:6187800 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:meshHeading | pubmed-meshheading:6187800-... | lld:pubmed |
| pubmed-article:6187800 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6187800 | pubmed:articleTitle | Cytochemical demonstration of adenylate cyclase with strontium chloride in the rat pancreas. | lld:pubmed |
| pubmed-article:6187800 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6187800 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
