| pubmed-article:6178738 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0450429 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0597979 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:6178738 | lifeskim:mentions | umls-concept:C1283195 | lld:lifeskim |
| pubmed-article:6178738 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:6178738 | pubmed:dateCreated | 1982-9-17 | lld:pubmed |
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| pubmed-article:6178738 | pubmed:abstractText | We identified two promoters for the beta-lactamase gene of plasmid pBR322. RNA isolated from bacteria containing pBR322 or RNA transcribed in vitro on pBR322 templates was hybridized to 5' end-labeled single-stranded plasmid probes (Berk, A. J., and Sharp, P. A. (1977) Cell 12, 721-732). Electrophoretic analysis of the nuclease S1 digestion products next to Maxam-Gilbert sequencing ladders closely defines the transcriptional initiation points. The natural promoter lies near the coding sequence of the beta-lactamase gene, initiating transcription at -35 bases before the ATG initiation codon, while a second promoter initiates at positions -244 and/or -245 (on the opposite side of the Eco RI site). This promoter overlaps the promoter transcribing in the opposite direction toward the tetracycline gene(s) and starts in the -10 region of that promoter. S1 mapping of procaryotic mRNA, transcribed in vivo, allows both an accurate identification of promoters and the analysis of their transcriptional regulation. | lld:pubmed |
| pubmed-article:6178738 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6178738 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6178738 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6178738 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6178738 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:6178738 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:6178738 | pubmed:author | pubmed-author:CateR LRL | lld:pubmed |
| pubmed-article:6178738 | pubmed:author | pubmed-author:BrosiusJJ | lld:pubmed |
| pubmed-article:6178738 | pubmed:author | pubmed-author:PerlmutterA... | lld:pubmed |
| pubmed-article:6178738 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6178738 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:6178738 | pubmed:volume | 257 | lld:pubmed |
| pubmed-article:6178738 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6178738 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6178738 | pubmed:pagination | 9205-10 | lld:pubmed |
| pubmed-article:6178738 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:6178738 | pubmed:meshHeading | pubmed-meshheading:6178738-... | lld:pubmed |
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| pubmed-article:6178738 | pubmed:meshHeading | pubmed-meshheading:6178738-... | lld:pubmed |
| pubmed-article:6178738 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6178738 | pubmed:articleTitle | Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro. | lld:pubmed |
| pubmed-article:6178738 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6178738 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6178738 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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