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pubmed-article:6143576pubmed:abstractTextScrapie hamster brains contain at least 5-10 micrograms of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differential centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.lld:pubmed
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pubmed-article:6143576pubmed:articleTitleA rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters.lld:pubmed
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