| pubmed-article:562751 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0012934 | lld:lifeskim |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0010843 | lld:lifeskim |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0025723 | lld:lifeskim |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:562751 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:562751 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:562751 | pubmed:dateCreated | 1978-1-27 | lld:pubmed |
| pubmed-article:562751 | pubmed:abstractText | Digestion of purified calf tymus satellite I (phi = 1.714 g/cm3) with a series of restriction enzymes shows that modification in this satellite occurs preferentially in the sequence C-G. This was also shown to be the case in the other satellites and in bulk chromosomal calf thymus DNA. Cloning of purified satellite I DNA in Escherichia coli makes sites, previously modified, available for cutting with certain restriction enzymes. All these 'new sites' contain the sequence C-G. High-resolution mass spectros-copy establishes that the satellites contain a low concentration of 5-methylcytosine. This infers that methylation which inhibits retriction enzyme cutting must occur preferentially in the sequence C-G. Hybridization of cRNA of cloned satellite I DNA with the satellites III (phi = 1.706 g/cm3) and IV (phi = 1.710 g/cm3) shows that there is no or little sequence homology between these satellites. Digestion of calf thymus satellite I DNA with endoR. EcoRI and subsequent hybridization studies with the fragments shows two EcoRI fragments in addition to the usual 1400-base-pair EcoRI repeat unit. | lld:pubmed |
| pubmed-article:562751 | pubmed:language | eng | lld:pubmed |
| pubmed-article:562751 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:562751 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:562751 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:562751 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:562751 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:562751 | pubmed:author | pubmed-author:BünemannHH | lld:pubmed |
| pubmed-article:562751 | pubmed:author | pubmed-author:GrotjahnLL | lld:pubmed |
| pubmed-article:562751 | pubmed:author | pubmed-author:GautierFF | lld:pubmed |
| pubmed-article:562751 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:562751 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:562751 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:562751 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:562751 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:562751 | pubmed:pagination | 175-83 | lld:pubmed |
| pubmed-article:562751 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:562751 | pubmed:year | 1977 | lld:pubmed |
| pubmed-article:562751 | pubmed:articleTitle | Analysis of calf-thymus satellite DNA: evidence for specific methylation of cytosine in C-G sequences. | lld:pubmed |
| pubmed-article:562751 | pubmed:publicationType | Journal Article | lld:pubmed |
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