| pubmed-article:3944117 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0026845 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0010531 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0769224 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:3944117 | lifeskim:mentions | umls-concept:C0067533 | lld:lifeskim |
| pubmed-article:3944117 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:3944117 | pubmed:dateCreated | 1986-3-12 | lld:pubmed |
| pubmed-article:3944117 | pubmed:abstractText | The association of regulatory subunits (RII) of Type II cAMP-dependent protein kinase from bovine cerebral cortex (RII-B) and bovine cardiac and skeletal muscle (RII-H) with specific binding proteins in bovine brain cytosol and purified brain microtubules was demonstrated using a solid phase binding assay. RII-binding proteins present in bovine cerebral cortex were immobilized on nitrocellulose filters after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Incubation of the filters with 32P-labeled regulatory subunits showed that both RII-B and RII-H interact with the 75,000-dalton calmodulin-binding protein (P75) and microtubule-associated protein 2 (MAP-2). However, significant differences in binding affinities and capacities were observed. RII-B displayed a higher affinity for P75 compared to RII-H while RII-H preferentially bound to MAP-2. Quantitation of radioactive RII bound to MAP-2 showed that MAP-2 bound 4-6 times more RII-H than RII-B. The differential binding affinities and capacities of RII-H and RII-B for MAP-2 were not affected by autophosphorylation since both phospho and dephospho forms of RII displayed the same binding characteristics. Competitive binding studies suggest that RII-H and RII-B bind to the same sites on MAP-2. The biochemical basis for the differential binding of RII-B and RII-H to the same sites of MAP-2 is unknown. However, other high affinity RII-binding proteins present in cerebral cortex (i.e. P75) might affect the affinity of RII-B for MAP-2. 32P-RI did not bind to P75 nor MAP-2 under the conditions used. | lld:pubmed |
| pubmed-article:3944117 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3944117 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3944117 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3944117 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:3944117 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3944117 | pubmed:author | pubmed-author:LeiserMM | lld:pubmed |
| pubmed-article:3944117 | pubmed:author | pubmed-author:RubinC SCS | lld:pubmed |
| pubmed-article:3944117 | pubmed:author | pubmed-author:ErlichmanJJ | lld:pubmed |
| pubmed-article:3944117 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3944117 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:3944117 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:3944117 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3944117 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3944117 | pubmed:pagination | 1904-8 | lld:pubmed |
| pubmed-article:3944117 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:meshHeading | pubmed-meshheading:3944117-... | lld:pubmed |
| pubmed-article:3944117 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3944117 | pubmed:articleTitle | Differential binding of the regulatory subunits (RII) of cAMP-dependent protein kinase II from bovine brain and muscle to RII-binding proteins. | lld:pubmed |
| pubmed-article:3944117 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3944117 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:3944117 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3944117 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3944117 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3944117 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3944117 | lld:pubmed |
