| pubmed-article:3930244 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0684336 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0032592 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0597295 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0032433 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0332162 | lld:lifeskim |
| pubmed-article:3930244 | lifeskim:mentions | umls-concept:C0333668 | lld:lifeskim |
| pubmed-article:3930244 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3930244 | pubmed:dateCreated | 1985-11-20 | lld:pubmed |
| pubmed-article:3930244 | pubmed:abstractText | Lymphocytes, exposed to mitogens in culture, show enhanced protein and RNA synthesis before the onset of DNA synthesis. Inhibition by DL-alpha-difluoromethylornithine of polyamine synthesis in phytohaemagglutinin-activated human lymphocytes resulted in a suppression of protein synthesis, which was evident before the initiation of DNA synthesis. The mitogen-induced increase in the incorporation of [3H]thymidine into DNA was subsequently inhibited in parallel with the activity of thymidine kinase in the polyamine-depleted cells. Ultraviolet absorbance measurement of the ribosomes after sucrose gradient centrifugation revealed a suppression of polyribosome formation that coincided with the decrease in the rate of protein synthesis. The disturbance in the polysomal profiles did not appear to be due to a shortage of mRNA, since the synthesis of poly(A)-rich mRNA was reduced less than that of rRNA after inhibiting polyamine synthesis. Entry of both the pre-existing and newly synthesized ribosomal subunits into polysomal structures was found to be impaired. These results thus suggest an important role for polyamines in the initiation step of protein synthesis. | lld:pubmed |
| pubmed-article:3930244 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3930244 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3930244 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3930244 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3930244 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:3930244 | pubmed:author | pubmed-author:HölttäEE | lld:pubmed |
| pubmed-article:3930244 | pubmed:author | pubmed-author:HoviTT | lld:pubmed |
| pubmed-article:3930244 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3930244 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:3930244 | pubmed:volume | 152 | lld:pubmed |
| pubmed-article:3930244 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3930244 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3930244 | pubmed:pagination | 229-37 | lld:pubmed |
| pubmed-article:3930244 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
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| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
| pubmed-article:3930244 | pubmed:meshHeading | pubmed-meshheading:3930244-... | lld:pubmed |
| pubmed-article:3930244 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3930244 | pubmed:articleTitle | Polyamine depletion results in impairment of polyribosome formation and protein synthesis before onset of DNA synthesis in mitogen-activated human lymphocytes. | lld:pubmed |
| pubmed-article:3930244 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3930244 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3930244 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3930244 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3930244 | lld:pubmed |
