pubmed-article:391803 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C0563594 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C1521721 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C1550518 | lld:lifeskim |
pubmed-article:391803 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:391803 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:391803 | pubmed:dateCreated | 1980-3-27 | lld:pubmed |
pubmed-article:391803 | pubmed:abstractText | Mutants of Escherichia coli K-12 defective in replication of F-like plasmids at a high temperature (42 degrees C) were found among threonine-independent (Thr+) revertants of a threonine-requiring F' stain after localized mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Transduction experiments with phage P1 permitted us to divide these mutations into two classes with respect to man location; some mutations were located between thr and ara at about 0.8 min, very close to maf-1 reported previously (Wada et al., J. Mol. Biol. 108:25-41, 1976 and the others probably were located between leu and azi at about 1.8 min. The former class of mutants designated mafA exhibited the same plasmid specificity as maf-1; replication of plasmids F and ColVB trp, but not R386 or R222, were affected at a high temperature. By contrast, the latter mutants designated mafB were defective in replication of nay of these plasmids at a high temperature. When a culture of mafA mutants carrying an F' plasmid was transferred from 30 to 42 degrees C, the plasmid replication as determined by incorporation of [3H]thymidine into covalently closed circular F DNA was markedly inhibited. Under certain conditions, the temperature shift-up caused severe growth inhibition of the mutant cells. Examination of merodiploids (mafA/FmafA+) for plasmid maintenance suggested that the two mafA mutations tested (mafA23 and mafA36) were both dominant, at least partially, over the wild-type mafA+ allele. These properties of the mafA mutants, manifested at the restrictive temperature, are similar to those previously reported for the maf-1 mutant. Taken together with other evidence it is likely that these mutations affect either the same gene (mafA) or a set of closely linked genes, playing a specific role in autonomous plasmid replication in E. coli. | lld:pubmed |
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pubmed-article:391803 | pubmed:language | eng | lld:pubmed |
pubmed-article:391803 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:391803 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:391803 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:391803 | pubmed:month | Dec | lld:pubmed |
pubmed-article:391803 | pubmed:issn | 0021-9193 | lld:pubmed |
pubmed-article:391803 | pubmed:author | pubmed-author:YuraTT | lld:pubmed |
pubmed-article:391803 | pubmed:author | pubmed-author:WadeOO | lld:pubmed |
pubmed-article:391803 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:391803 | pubmed:volume | 140 | lld:pubmed |
pubmed-article:391803 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:391803 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:391803 | pubmed:pagination | 864-73 | lld:pubmed |
pubmed-article:391803 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:391803 | pubmed:meshHeading | pubmed-meshheading:391803-F... | lld:pubmed |
pubmed-article:391803 | pubmed:year | 1979 | lld:pubmed |
pubmed-article:391803 | pubmed:articleTitle | Escherichia coli mutants incapable of supporting replication of F-like plasmids at high temperature: isolation and characterization of mafA and mafB mutants. | lld:pubmed |
pubmed-article:391803 | pubmed:publicationType | Journal Article | lld:pubmed |
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