| pubmed-article:3856331 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C0205102 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C0039667 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C0011155 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C1140606 | lld:lifeskim |
| pubmed-article:3856331 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:3856331 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3856331 | pubmed:dateCreated | 1985-4-11 | lld:pubmed |
| pubmed-article:3856331 | pubmed:abstractText | Dihydrofolate reductase-deficient mutants of Chinese hamster ovary cells have been selected based on their inability to bind a fluorescent derivative of methotrexate, a substrate analog. Nonfluorescent mutant cells were isolated from mutagenized populations using a fluorescence-activated cell sorter. After multiple rounds of sorting plus regrowth, the mutant cell frequency was increased from an initial 10(-5) to greater than 0.9. This use of the cell sorter to isolate mutants deficient in an internal protein should be applicable to any gene product that is able to bind a fluorescent ligand tightly. The method has the advantage of allowing the screening of large numbers of cells and of selecting for partially expressed phenotypes. | lld:pubmed |
| pubmed-article:3856331 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3856331 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3856331 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3856331 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3856331 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3856331 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3856331 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:3856331 | pubmed:issn | 0740-7750 | lld:pubmed |
| pubmed-article:3856331 | pubmed:author | pubmed-author:ChasinL ALA | lld:pubmed |
| pubmed-article:3856331 | pubmed:author | pubmed-author:UrlaubGG | lld:pubmed |
| pubmed-article:3856331 | pubmed:author | pubmed-author:McDowellJJ | lld:pubmed |
| pubmed-article:3856331 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3856331 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:3856331 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3856331 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3856331 | pubmed:pagination | 71-7 | lld:pubmed |
| pubmed-article:3856331 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:meshHeading | pubmed-meshheading:3856331-... | lld:pubmed |
| pubmed-article:3856331 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3856331 | pubmed:articleTitle | Use of fluorescence-activated cell sorter to isolate mutant mammalian cells deficient in an internal protein, dihydrofolate reductase. | lld:pubmed |
| pubmed-article:3856331 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3856331 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:3856331 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3856331 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3856331 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3856331 | lld:pubmed |
