| pubmed-article:3768308 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C0006657 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C0042872 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:3768308 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:3768308 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:3768308 | pubmed:dateCreated | 1986-11-24 | lld:pubmed |
| pubmed-article:3768308 | pubmed:abstractText | It is well recognized that the vitamin D binding protein (DBP) is important for the transport of vitamin D, 25-hydroxyvitamin D (25-OH-D), and its metabolites. In an attempt to better understand the molecular-binding properties of this ubiquitous protein, we designed and synthesized a photoaffinity analogue of 25-OH-D3 and its radiolabeled counterpart. This analogue, 25-hydroxyvitamin D3 3 beta-[N-(4-azido-2-nitrophenyl)glycinate] (25-OH-D3-ANG), was recognized by the rat DBP and was about 10 times less active than 25-OH-D3 in terms of binding. Incubation of [3H]25-OH-D3 or [3H]25-OH-D3-ANG with rat DBP revealed that both compounds were specifically bound to a protein with a sedimentation coefficient of 4.1 S. Each was displaced with a 500-fold excess of 25-OH-D3. When [3H]25-OH-D3-ANG was exposed to UV radiation in the presence of rat DBP followed by the addition of a 500-fold excess of 25-OH-D3, there was no displacement of tritium from the 4.1S peak. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiographic analysis of [3H]25-OH-D3-ANG exposed to UV radiation in the presence of rat DBP followed by the addition of a 500-fold excess of 25-OH-D3 revealed one major band with a molecular weight of 52 000. These data provide strong evidence that [3H]25-OH-D3-ANG was covalently linked to the rat DBP. This photoaffinity probe should provide a valuable tool for the analysis of the binding site on this transport protein. | lld:pubmed |
| pubmed-article:3768308 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3768308 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3768308 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3768308 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3768308 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:3768308 | pubmed:author | pubmed-author:RazSS | lld:pubmed |
| pubmed-article:3768308 | pubmed:author | pubmed-author:HolickM FMF | lld:pubmed |
| pubmed-article:3768308 | pubmed:author | pubmed-author:HolickS ASA | lld:pubmed |
| pubmed-article:3768308 | pubmed:author | pubmed-author:HanafinNN | lld:pubmed |
| pubmed-article:3768308 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3768308 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:3768308 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:3768308 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3768308 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3768308 | pubmed:pagination | 4729-33 | lld:pubmed |
| pubmed-article:3768308 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:meshHeading | pubmed-meshheading:3768308-... | lld:pubmed |
| pubmed-article:3768308 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3768308 | pubmed:articleTitle | Photoaffinity labeling of the rat plasma vitamin D binding protein with [26,27-3H]-25-hydroxyvitamin D3 3 beta-[N-(4-azido-2-nitrophenyl)glycinate]. | lld:pubmed |
| pubmed-article:3768308 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3768308 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3768308 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3768308 | lld:pubmed |
