| pubmed-article:3711022 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0085470 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0205103 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0030011 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C1549781 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C0772162 | lld:lifeskim |
| pubmed-article:3711022 | lifeskim:mentions | umls-concept:C1481566 | lld:lifeskim |
| pubmed-article:3711022 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3711022 | pubmed:dateCreated | 1986-7-17 | lld:pubmed |
| pubmed-article:3711022 | pubmed:abstractText | Pseudomonas putida BG1 was isolated from soil by enrichment with p-toluate and selection for growth with p-xylene. Other hydrocarbons that served as growth substrates were toluene, m-xylene, 3-ethyltoluene, and 1,2,4-trimethylbenzene. The enzymes responsible for growth on these substrates are encoded by a large plasmid with properties similar to those of TOL plasmids isolated from other strains of Pseudomonas. Treatment of P. putida BG1 with nitrosoguanidine led to the isolation of a mutant strain which, when grown with fructose, oxidized both p-xylene and p-toluate to (-)-cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylic acid (cis-p-toluate diol). The structure of the diol was determined by conventional chemical techniques including identification of the products formed by acid-catalyzed dehydration and characterization of a methyl ester derivative. The cis-relative stereochemistry of the hydroxyl groups was determined by the isolation and characterization of an isopropylidene derivative. p-Xylene-grown cells contained an inducible NAD+-dependent dehydrogenase which formed catechols from cis-p-toluate diol and the analogous acid diols formed from the other hydrocarbon substrates listed above. The catechols were converted to meta ring fission products by an inducible catechol-2,3-dioxygenase which was partially purified from p-xylene-grown cells of P. putida BG1. | lld:pubmed |
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| pubmed-article:3711022 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3711022 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3711022 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3711022 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3711022 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3711022 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3711022 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:3711022 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:3711022 | pubmed:author | pubmed-author:GibsonD TDT | lld:pubmed |
| pubmed-article:3711022 | pubmed:author | pubmed-author:McCombieW RWR | lld:pubmed |
| pubmed-article:3711022 | pubmed:author | pubmed-author:KwartL DLD | lld:pubmed |
| pubmed-article:3711022 | pubmed:author | pubmed-author:WhitedG MGM | lld:pubmed |
| pubmed-article:3711022 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3711022 | pubmed:volume | 166 | lld:pubmed |
| pubmed-article:3711022 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3711022 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3711022 | pubmed:pagination | 1028-39 | lld:pubmed |
| pubmed-article:3711022 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:meshHeading | pubmed-meshheading:3711022-... | lld:pubmed |
| pubmed-article:3711022 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3711022 | pubmed:articleTitle | Identification of cis-diols as intermediates in the oxidation of aromatic acids by a strain of Pseudomonas putida that contains a TOL plasmid. | lld:pubmed |
| pubmed-article:3711022 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3711022 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3711022 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
