| pubmed-article:3680408 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C1185738 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C0034627 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C0034809 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C1823517 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:3680408 | lifeskim:mentions | umls-concept:C1551086 | lld:lifeskim |
| pubmed-article:3680408 | pubmed:dateCreated | 1988-1-15 | lld:pubmed |
| pubmed-article:3680408 | pubmed:abstractText | The Stokes radius of the rat liver glucocorticoid receptor was determined using TSK G3000SW and TSK G4000SW high-performance size-exclusion columns. The accuracy of the calibration graph for proteins larger than 6 nm on the TSK G4000SW column allowed the resolution of a heterogeneous structure for the cytosolic untransformed receptor, giving two forms with Rs values of 8.3 and 7.1 nm, whereas the transformed receptor elutes with an Rs value of 4.7-5.3 nm. The 8.3 nm form was not observed for the highly purified untransformed receptor. Parallel analyses of the cytosolic untransformed receptor on conventional gravity-fed Bio-Gel A 1.5-m or Ultrogel AcA-22 size-exclusion columns could not resolve two components. The resolution efficiencies of high-performance size-exclusion chromatography and open-column size-exclusion chromatography were compared. Further, owing to its rapidity, high-performance chromatography allowed the characterization of steroid-receptor complexes having half-lives as short as 90 min and very unstable receptor forms could be detected. Specific applications are considered, such as the resort to a small TSK GSWP guard column for the rapid separation of affinity-purified [3H]TA-receptor complexes from free eluting steroid, and to a preparative TSK G4000SW column for the fractionation of significant amounts of the two untransformed receptor forms. | lld:pubmed |
| pubmed-article:3680408 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3680408 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3680408 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3680408 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3680408 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3680408 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3680408 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3680408 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:3680408 | pubmed:author | pubmed-author:DautrevauxMM | lld:pubmed |
| pubmed-article:3680408 | pubmed:author | pubmed-author:FormstecherPP | lld:pubmed |
| pubmed-article:3680408 | pubmed:author | pubmed-author:LefebvrePP | lld:pubmed |
| pubmed-article:3680408 | pubmed:author | pubmed-author:SablonniereBB | lld:pubmed |
| pubmed-article:3680408 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3680408 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:3680408 | pubmed:volume | 403 | lld:pubmed |
| pubmed-article:3680408 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3680408 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3680408 | pubmed:pagination | 183-96 | lld:pubmed |
| pubmed-article:3680408 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:meshHeading | pubmed-meshheading:3680408-... | lld:pubmed |
| pubmed-article:3680408 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3680408 | pubmed:articleTitle | Improved Stokes radius measurement of the glucocorticoid receptor using TSK G4000SW and TSK G3000SW high-performance size-exclusion columns. Analytical and preparative applications. | lld:pubmed |
| pubmed-article:3680408 | pubmed:affiliation | Laboratoire de Biochimie Structurale, Faculté de Médecine, Lille, France. | lld:pubmed |
| pubmed-article:3680408 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3680408 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
