| pubmed-article:3680292 | pubmed:abstractText | We have previously reported the presence of GM2 as the major ganglioside in the roe of striped mullet, Mugil cephalus, (Li, Y.-T., Hirabayashi, Y., DeGasperi, R., Yu, R. K., Ariga, T., Koerner, T. A. W., and Li, S.-C. (1984) J. Biol. Chem. 259, 8980-8985). In addition to GM2, mullet roe also contain a series of gangliosides with thin-layer chromatographic mobilities slower than GM2. Besides enzymatic hydrolysis and NMR spectroscopy, we have employed the thin-layer chromatography overlay technique using a human monoclonal IgM antibody which recognizes the GM2 epitope to study the nature of these gangliosides. Using these methods we have isolated and characterized three novel mullet roe gangliosides with the following structures: (Formula: see text). These three gangliosides all contain neolacto-series sugar chains. However, the unique feature of gangliosides 5 and 10 is that the terminal portion of the sugar chain is of the ganglio-series while the internal portion is of the neolacto series structure. Due to the substitution of a GalNAc on the internal Gal in 9 and 10 in the inner core, these two gangliosides also contain the gangliotriaosyl structure. Thus, the sugar chains in these gangliosides are of novel type and can be considered a hybrid between the two series which can be defined as the neolacto-ganglio series. | lld:pubmed |
