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pubmed-article:3552119pubmed:abstractTextTo elucidate the differences between neurons of epileptogenic animals and those of normal animals, cellular characteristics of neurons of mutant strain El mice which are highly susceptible to seizures were investigated using immunocytochemical techniques. In neurons of 3-day primary cultures, the control ddY mouse neurons showed dividing stages in about 0.2% of neurofilament (NF)-positive neurons, whereas no dividing neurons were observed among the NF-positive El mouse neurons. In 7-day cultures, localization of GD3 ganglioside in the proliferating control ddY mouse neurons was observed, but there was no GD3 ganglioside in the mutant El mouse neuron. The content of GD3 ganglioside detected by high-performance thin-layer chromatography of El mouse cultured cells was ca. 1/4 of that of ddy mice. These findings suggest that neurons of the El mouse are differentiated earlier than those of the control ddY mouse.lld:pubmed
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pubmed-article:3552119pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3552119pubmed:articleTitleCharacteristics of primary cultured neurons from embryonic mutant El mouse cerebral cortex.lld:pubmed
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pubmed-article:3552119pubmed:publicationTypeComparative Studylld:pubmed