| pubmed-article:344137 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:344137 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:344137 | lifeskim:mentions | umls-concept:C0042444 | lld:lifeskim |
| pubmed-article:344137 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:344137 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:344137 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:344137 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:344137 | pubmed:dateCreated | 1978-5-17 | lld:pubmed |
| pubmed-article:344137 | pubmed:abstractText | In vitro recombination techniques were used to construct a new cloning vehicle, pBR322. This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI. Survival of Escherichia coli strain X1776 containing pBR313 and pBR322 as a function of thymine and diaminopimelic acid (DAP) starvation and sensitivity to bile salts was found to be equivalent to the non-plasmid containing strain. Conjugal transfer of these plasmids in bi- and triparental matings were significantly reduced or undetectable relative to the plasmid ColE1. | lld:pubmed |
| pubmed-article:344137 | pubmed:language | eng | lld:pubmed |
| pubmed-article:344137 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:344137 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:344137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:344137 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:344137 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:HeynekerH LHL | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:BolivarFF | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:FalkowSS | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:RodriguezR... | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:BoyerH WHW | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:BetlachM CMC | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:CrosaJ HJH | lld:pubmed |
| pubmed-article:344137 | pubmed:author | pubmed-author:GreeneP JPJ | lld:pubmed |
| pubmed-article:344137 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:344137 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:344137 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:344137 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:344137 | pubmed:pagination | 95-113 | lld:pubmed |
| pubmed-article:344137 | pubmed:dateRevised | 2009-2-4 | lld:pubmed |
| pubmed-article:344137 | pubmed:meshHeading | pubmed-meshheading:344137-E... | lld:pubmed |
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| pubmed-article:344137 | pubmed:meshHeading | pubmed-meshheading:344137-D... | lld:pubmed |
| pubmed-article:344137 | pubmed:year | 1977 | lld:pubmed |
| pubmed-article:344137 | pubmed:articleTitle | Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. | lld:pubmed |
| pubmed-article:344137 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:344137 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:344137 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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