| pubmed-article:3392017 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3392017 | lifeskim:mentions | umls-concept:C0026046 | lld:lifeskim |
| pubmed-article:3392017 | lifeskim:mentions | umls-concept:C1444783 | lld:lifeskim |
| pubmed-article:3392017 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:3392017 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:3392017 | pubmed:dateCreated | 1988-8-19 | lld:pubmed |
| pubmed-article:3392017 | pubmed:abstractText | Evidence for a slowly dissociating tubulin-GTP cap at microtubule ends was derived from observation of a delay for attaining a maximum disassembly rate, after the temperature of steady state microtubules was rapidly decreased from 36 to 34 degrees C. The possibility that the microtubules were capped by a single tubulin-GTP subunit on each subhelix was ruled out, by comparison of the disassembly kinetics following a temperature decrease and dilution. The existence of a subpopulation of microtubules that underwent irreversible or near irreversible disassembly was demonstrated by a 30-s lag for attainment of a maximum assembly rate, after steady state microtubules were shifted from 34 to 36 degrees C. A dynamic instability model predicts that a maximum assembly rate will be delayed until disappearance of a subpopulation of microtubules that disassemble before being recapped. Analysis indicates that the 30-s lag resulted because approximately 2% of the mass in the steady state microtubule population was uncapped and disassembling and not readily recapped. The half-time for recapping of disassembling microtubules, by addition of tubulin-GTP subunits to ends, was equal to or greater than 20 s. Since tubulin-GDP dissociated from microtubules at a rate of about 4500 s-1, slow recapping resulted in dramatic shortening of disassembling microtubules. | lld:pubmed |
| pubmed-article:3392017 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392017 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3392017 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392017 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3392017 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392017 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392017 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392017 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392017 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3392017 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3392017 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3392017 | pubmed:author | pubmed-author:ShanksJJ | lld:pubmed |
| pubmed-article:3392017 | pubmed:author | pubmed-author:CaplowMM | lld:pubmed |
| pubmed-article:3392017 | pubmed:author | pubmed-author:RuhlenR LRL | lld:pubmed |
| pubmed-article:3392017 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3392017 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:3392017 | pubmed:volume | 263 | lld:pubmed |
| pubmed-article:3392017 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3392017 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3392017 | pubmed:pagination | 10344-52 | lld:pubmed |
| pubmed-article:3392017 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
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| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:meshHeading | pubmed-meshheading:3392017-... | lld:pubmed |
| pubmed-article:3392017 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3392017 | pubmed:articleTitle | Temperature-jump studies of microtubule dynamic instability. | lld:pubmed |
| pubmed-article:3392017 | pubmed:affiliation | Department of Biochemistry, University of North Carolina, Chapel Hill 27599-7260. | lld:pubmed |
| pubmed-article:3392017 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3392017 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3392017 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3392017 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3392017 | lld:pubmed |
