| pubmed-article:3388291 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C0138741 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C0205242 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C0019536 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C0015522 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C1527121 | lld:lifeskim |
| pubmed-article:3388291 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:3388291 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3388291 | pubmed:dateCreated | 1988-7-29 | lld:pubmed |
| pubmed-article:3388291 | pubmed:abstractText | A method for the quantitative assay of native single chain and kallikrein cleaved two-chain high molecular weight (HMW)-kininogen in plasma is described. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of whole plasma is followed by electrotransfer of the electropherogram to nitrocellulose membranes and detection of the blotted HMW-kininogen with its physiologic ligands, radiolabeled plasma prekallikrein or radiolabeled factor XI. Using unreduced SDS-PAGE cleaved two-chain HMW-kininogen (Mr approximately 107,000 and 95,000), is electrophoretically separated from uncleaved single chain HMW-kininogen (Mr approximately 150,000). Counting the radioactivity of the nitrocellulose pieces corresponding to cleaved HMW-kininogen permits its quantitative measurement by comparison with standards consisting of decreasing amounts of fully dextran sulfate activated normal human plasma. Single chain HMW-kininogen is similarly assayed using reduced SDS-PAGE and unactivated normal human plasma standards. This technique is highly specific and sensitive to about 50 ng of either cleaved or uncleaved HMW-kininogen. Varying amounts of cleaved HMW-kininogen were found in a small series of plasmas from patients suffering from various inflammatory conditions. Higher levels of in vivo cleaved HMW-kininogen were observed during acute attacks of hereditary angioedema due to Cl-inhibitor deficiency. This technique may be useful for the assessment of the degree of in vitro or in vivo activation of the contact system. | lld:pubmed |
| pubmed-article:3388291 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3388291 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3388291 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3388291 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3388291 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3388291 | pubmed:issn | 0340-6245 | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:CurdJ GJG | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:GriffinJ HJH | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:LämmleBB | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:BerrettiniMM | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:SchwarzH PHP | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:ZurawB LBL | lld:pubmed |
| pubmed-article:3388291 | pubmed:author | pubmed-author:HeebM JMJ | lld:pubmed |
| pubmed-article:3388291 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3388291 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:3388291 | pubmed:volume | 59 | lld:pubmed |
| pubmed-article:3388291 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3388291 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3388291 | pubmed:pagination | 151-61 | lld:pubmed |
| pubmed-article:3388291 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:meshHeading | pubmed-meshheading:3388291-... | lld:pubmed |
| pubmed-article:3388291 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3388291 | pubmed:articleTitle | Detection and quantitation of cleaved and uncleaved high molecular weight kininogen in plasma by ligand blotting with radiolabeled plasma prekallikrein or factor XI. | lld:pubmed |
| pubmed-article:3388291 | pubmed:affiliation | Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, CA 92037. | lld:pubmed |
| pubmed-article:3388291 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3388291 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3388291 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3388291 | lld:pubmed |
