| pubmed-article:3365772 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3365772 | lifeskim:mentions | umls-concept:C0013710 | lld:lifeskim |
| pubmed-article:3365772 | lifeskim:mentions | umls-concept:C0015914 | lld:lifeskim |
| pubmed-article:3365772 | lifeskim:mentions | umls-concept:C0025186 | lld:lifeskim |
| pubmed-article:3365772 | lifeskim:mentions | umls-concept:C0026255 | lld:lifeskim |
| pubmed-article:3365772 | lifeskim:mentions | umls-concept:C0026046 | lld:lifeskim |
| pubmed-article:3365772 | lifeskim:mentions | umls-concept:C0599577 | lld:lifeskim |
| pubmed-article:3365772 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3365772 | pubmed:dateCreated | 1988-6-14 | lld:pubmed |
| pubmed-article:3365772 | pubmed:abstractText | The sequential changes in the distribution of microtubules during germinal vesicle breakdown (GVBD), fertilization, and mitosis were investigated with antitubulin indirect immunofluorescence microscopy in several species of ascidian eggs (Molgula occidentalis, Ciona savignyi, and Halocynthia roretzi). These alterations in microtubule patterns were also correlated with observed cytoplasmic movements. A cytoplasmic latticework of microtubules was observed throughout meiosis. The unfertilized egg of M. occidentalis had a small meiotic spindle with wide poles; the poles became focused after egg activation. The other two species had more typical meiotic spindles before fertilization. At fertilization, a sperm aster first appeared near the cortex close to the vegetal pole. It enlarged into an unusual asymmetric aster associated with the egg cortex. The sperm aster rapidly grew after the formation of the second polar body, and it was displaced as far as the equatorial region, corresponding to the site of the myoplasmic crescent, the posterior half of the egg. The female pronucleus migrated to the male pronucleus at the center of the sperm aster. The microtubule latticework and the sperm aster disappeared towards the end of first interphase with only a small bipolar structure remaining until first mitosis. At mitosis the asters enlarged tremendously, while the mitotic spindle remained remarkably small. The two daughter nuclei remained near the site of cleavage even after division was complete. These results document the changes in microtubule patterns during maturation in Ascidian oocytes, demonstrate that the sperm contributes the active centrosome at fertilization, and reveal the presence of a mitotic apparatus at first division which has an unusually small spindle and huge asters. | lld:pubmed |
| pubmed-article:3365772 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3365772 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3365772 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3365772 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3365772 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3365772 | pubmed:issn | 0886-1544 | lld:pubmed |
| pubmed-article:3365772 | pubmed:author | pubmed-author:SawadaTT | lld:pubmed |
| pubmed-article:3365772 | pubmed:author | pubmed-author:SchattenGG | lld:pubmed |
| pubmed-article:3365772 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3365772 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:3365772 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3365772 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3365772 | pubmed:pagination | 219-30 | lld:pubmed |
| pubmed-article:3365772 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:meshHeading | pubmed-meshheading:3365772-... | lld:pubmed |
| pubmed-article:3365772 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3365772 | pubmed:articleTitle | Microtubules in ascidian eggs during meiosis, fertilization, and mitosis. | lld:pubmed |
| pubmed-article:3365772 | pubmed:affiliation | Department of Anatomy, Yamaguchi University School of Medicine, Ube, Japan. | lld:pubmed |
| pubmed-article:3365772 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3365772 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3365772 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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