| pubmed-article:3289103 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C0001675 | lld:lifeskim |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C0596981 | lld:lifeskim |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C1882687 | lld:lifeskim |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C0332325 | lld:lifeskim |
| pubmed-article:3289103 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:3289103 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3289103 | pubmed:dateCreated | 1988-7-19 | lld:pubmed |
| pubmed-article:3289103 | pubmed:abstractText | Maintaining viability in cardiac myocytes isolated from adult rats using collagenase is difficult in Ca-containing media due to cell damage that occurs on reintroduction of Ca after perfusing the heart with the Ca-free medium needed to isolate myocytes with collagenase. Recently it has been proposed that Ca-free perfusion of isolated rabbit interventricular septa leads to cellular Na overload which, on reintroducing Ca, produces influx of toxic concentrations of Ca due to the Na/Ca exchange mechanism in the sarcolemma. We have found that replacing a portion of the 118 mM NaCl in the Ca-free perfusion medium with 69 mM LiCl dramatically increased the proportion of Ca-tolerant cardiac myocytes isolated from adult male rats with collagenase. Myocyte viability was maintained over a four hour period of incubation at 37 degrees C in 1 mM Ca. | lld:pubmed |
| pubmed-article:3289103 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3289103 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3289103 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3289103 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3289103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3289103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3289103 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3289103 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3289103 | pubmed:issn | 0034-5164 | lld:pubmed |
| pubmed-article:3289103 | pubmed:author | pubmed-author:WeissA JAJ | lld:pubmed |
| pubmed-article:3289103 | pubmed:author | pubmed-author:KocsisJ JJJ | lld:pubmed |
| pubmed-article:3289103 | pubmed:author | pubmed-author:KhetarpalV... | lld:pubmed |
| pubmed-article:3289103 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3289103 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:3289103 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3289103 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3289103 | pubmed:pagination | 117-20 | lld:pubmed |
| pubmed-article:3289103 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:meshHeading | pubmed-meshheading:3289103-... | lld:pubmed |
| pubmed-article:3289103 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3289103 | pubmed:articleTitle | An improved method for isolating Ca2+-resistant myocytes from the adult rat heart. | lld:pubmed |
| pubmed-article:3289103 | pubmed:affiliation | Department of Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107. | lld:pubmed |
| pubmed-article:3289103 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3289103 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
