| pubmed-article:3192212 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0012931 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0237401 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C1512899 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0008643 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0872147 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C1621812 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0444498 | lld:lifeskim |
| pubmed-article:3192212 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:3192212 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3192212 | pubmed:dateCreated | 1989-1-6 | lld:pubmed |
| pubmed-article:3192212 | pubmed:abstractText | A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported. DNA inserts from a single chromosomal library are labeled with biotin and partially preannealed with a titrated amount of total human genomic DNA prior to hybridization with cellular or chromosomal preparations. The cross-hybridization of repetitive sequences to nontargeted chromosomes can be markedly suppressed under appropriate preannealing conditions. The remaining single-stranded DNA is hybridized to specimens of interest and detected with fluorescent or enzyme-labeled avidin conjugates following post-hybridization washes. DNA inserts from recombinant libraries for chromosomes 1, 4, 7, 8, 13, 14, 18, 20, 21, 22, and X were assessed for their ability to decorate specifically their cognate chromosome; most libraries proved to be highly specific. Quantitative densitometric analyses indicated that the ratio of specific to nonspecific hybridization signal under optimal preannealing conditions was at least 8:1. Interphase nuclei showed a cohesive territorial organization of chromosomal domains, and laser-scanning confocal fluorescence microscopy was used to aid the 3-D visualization of these domains. This method should be useful for both karyotypic studies and for the analysis of chromosome topography in interphase cells. | lld:pubmed |
| pubmed-article:3192212 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3192212 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3192212 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3192212 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3192212 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3192212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3192212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3192212 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3192212 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:3192212 | pubmed:issn | 0340-6717 | lld:pubmed |
| pubmed-article:3192212 | pubmed:author | pubmed-author:WardD CDC | lld:pubmed |
| pubmed-article:3192212 | pubmed:author | pubmed-author:ManuelidisLL | lld:pubmed |
| pubmed-article:3192212 | pubmed:author | pubmed-author:CremerTT | lld:pubmed |
| pubmed-article:3192212 | pubmed:author | pubmed-author:LichterPP | lld:pubmed |
| pubmed-article:3192212 | pubmed:author | pubmed-author:BordenJJ | lld:pubmed |
| pubmed-article:3192212 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3192212 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:3192212 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3192212 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3192212 | pubmed:pagination | 224-34 | lld:pubmed |
| pubmed-article:3192212 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:meshHeading | pubmed-meshheading:3192212-... | lld:pubmed |
| pubmed-article:3192212 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3192212 | pubmed:articleTitle | Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. | lld:pubmed |
| pubmed-article:3192212 | pubmed:affiliation | Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510. | lld:pubmed |
| pubmed-article:3192212 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3192212 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3192212 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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