| pubmed-article:3185543 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0026845 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0018787 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0521324 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0002240 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:3185543 | lifeskim:mentions | umls-concept:C0205214 | lld:lifeskim |
| pubmed-article:3185543 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:3185543 | pubmed:dateCreated | 1988-12-21 | lld:pubmed |
| pubmed-article:3185543 | pubmed:abstractText | The skeletal and cardiac alpha-actin genes are coexpressed in muscle development but exhibit distinctive tissue-specific patterns of expression. We used an in vivo competition assay and an in vitro electrophoretic mobility shift assay to demonstrate that both genes interact with a common trans-acting factor(s). However, there was at least one gene-specific cis-acting sequence in the skeletal alpha-actin gene that interacted with a trans-acting factor which was not rate limiting in the expression of the cardiac alpha-actin gene. The common factor(s) interacted with several cis-acting regions that corresponded to sequences that are required for the transcriptional modulation of these sarcomeric alpha-actin genes in muscle cells. These regulatory regions contained the sequence motif CC(A + T-rich)6GG, which is known as a CArG box. Results of in vivo competition assays demonstrated that the factor(s) bound by the skeletal alpha-actin gene is also essential for the maximal activity of the cardiac alpha-actin, simian virus 40 (SV40), alpha 2(I)-collagen, and the beta-actin promoters in muscle cells. In contrast, fibroblastic cells contained functionally distinct transcription factor(s) that were used by the SV40 enhancer but that did not interact with the sarcomeric alpha-actin cis-acting sequences. The existence of functionally different factors in these cell types may explain the myogenic specificity of these sarcomeric alpha-actin genes. Results of in vitro studies suggested that both the sarcomeric alpha-actin genes interact with the CArG box-binding factor CBF and that the skeletal alpha-actin promoter contains multiple CBF-binding sites. In contrast, CBF did not interact in vitro with a classical CAAT box, the SV40 enhancer, or a linker scanner mutation of an alpha-actin CArG box. Furthermore, methylation interference and DNase I footprinting assays demonstrated the precise sites of interaction of CBF with three CArG motifs at positions -98, -179, and -225 in the human skeletal alpha-actin gene. | lld:pubmed |
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| pubmed-article:3185543 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3185543 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3185543 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3185543 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3185543 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3185543 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:3185543 | pubmed:author | pubmed-author:KedesLL | lld:pubmed |
| pubmed-article:3185543 | pubmed:author | pubmed-author:MuscatG EGE | lld:pubmed |
| pubmed-article:3185543 | pubmed:author | pubmed-author:GustafsonT... | lld:pubmed |
| pubmed-article:3185543 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3185543 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:3185543 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3185543 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3185543 | pubmed:pagination | 4120-33 | lld:pubmed |
| pubmed-article:3185543 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:3185543 | pubmed:meshHeading | pubmed-meshheading:3185543-... | lld:pubmed |
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| pubmed-article:3185543 | pubmed:meshHeading | pubmed-meshheading:3185543-... | lld:pubmed |
| pubmed-article:3185543 | pubmed:meshHeading | pubmed-meshheading:3185543-... | lld:pubmed |
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| pubmed-article:3185543 | pubmed:meshHeading | pubmed-meshheading:3185543-... | lld:pubmed |
| pubmed-article:3185543 | pubmed:meshHeading | pubmed-meshheading:3185543-... | lld:pubmed |
| pubmed-article:3185543 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3185543 | pubmed:articleTitle | A common factor regulates skeletal and cardiac alpha-actin gene transcription in muscle. | lld:pubmed |
