| pubmed-article:3138386 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0004112 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0002607 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0017725 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0030011 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0244104 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:3138386 | lifeskim:mentions | umls-concept:C0106070 | lld:lifeskim |
| pubmed-article:3138386 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:3138386 | pubmed:dateCreated | 1988-10-19 | lld:pubmed |
| pubmed-article:3138386 | pubmed:abstractText | Both ammonia and beta-methylene-DL-aspartate (beta-MA), an irreversible inhibitor of aspartate aminotransferase activity and thus of the malate-aspartate shuttle, were found previously to decrease oxidative metabolism in cerebral cortex slices. In the present work, the possibility that ammonia and beta-MA affect energy metabolism by a common mechanism (i.e., via inhibition of the malate-aspartate shuttle) was investigated using primary cultures of neurons and astrocytes. Incubation of astrocytes for 30 min with 5 mM beta-MA resulted in a decreased production of 14CO2 from [U-14C]glucose, but did not affect 14CO2 production from [2-14C]pyruvate. Conversely, incubation of astrocytes with 3 mM ammonium chloride resulted in decreased 14CO2 production from [2-14C]pyruvate, but 14CO2 production from [U-14C]glucose was not significantly affected. Ammonium chloride had no significant effect on 14CO2 production from either [U-14C]glucose or [2-14]pyruvate by neurons. However, incubation of neurons with beta-MA or beta-MA plus ammonium chloride resulted in a approximately 45% decrease of 14CO2 production from both [U-14C]glucose and [2-14C]pyruvate. A 2-h incubation of astrocytes with beta-MA resulted in no change in ATP levels, but a 35% decrease in phosphocreatine. Similar treatment of neurons resulted in greater than 50% decrease in ATP, but had little effect on phosphocreatine. beta-MA also caused a decrease in glutamate and aspartate content of neurons, but not of astrocytes. The different metabolic responses of neurons and astrocytes towards beta-MA were probably not due to a differential inhibition of aspartate aminotransferase which was inhibited by approximately 45% in astrocytes and by approximately 55% in neurons. | lld:pubmed |
| pubmed-article:3138386 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3138386 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3138386 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3138386 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3138386 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:3138386 | pubmed:author | pubmed-author:CooperA JAJ | lld:pubmed |
| pubmed-article:3138386 | pubmed:author | pubmed-author:HertzLL | lld:pubmed |
| pubmed-article:3138386 | pubmed:author | pubmed-author:FitzpatrickS... | lld:pubmed |
| pubmed-article:3138386 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3138386 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:3138386 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3138386 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3138386 | pubmed:pagination | 1197-203 | lld:pubmed |
| pubmed-article:3138386 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3138386 | pubmed:meshHeading | pubmed-meshheading:3138386-... | lld:pubmed |
| pubmed-article:3138386 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3138386 | pubmed:articleTitle | Effects of ammonia and beta-methylene-DL-aspartate on the oxidation of glucose and pyruvate by neurons and astrocytes in primary culture. | lld:pubmed |
| pubmed-article:3138386 | pubmed:affiliation | Department of Neurology, Cornell University Medical College, New York, NY 10021. | lld:pubmed |
| pubmed-article:3138386 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3138386 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3138386 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
