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pubmed-article:3075143pubmed:abstractTextTo elucidate whether testosterone (T) replacement to castrated male rats may promote luteinizing hormone-releasing hormone (LH-RH) biosynthesis in the hypothalamus, an RNA-blot hybridization assay was employed. Poly(A) mRNA fractions from hypothalami (40-50) of intact, castrated plus vehicle, and castrated plus T group were isolated, blotted on nitrocellulose paper and hybridized with 32P-end labelled LH-RH oligonucleotides (29 mer) which is complementary to the rat LH-RH mRNA. LH-RH-like mRNA level markedly attenuated 2 weeks following castration and T replacement significantly increased LH-RH-like mRNA level, comparable to about 80% of that observed in intact male rats. In addition, LH-RH contents and release from hypothalami were studied, to examine the correlation with LH-RH gene expression and secretory activity of LH-RH. Castration significantly reduced LH-RH contents and LH-RH release in vitro. T replacement restored LH-RH contents and release comparable to those shown in the intact group. This study demonstrates for the first time that T replacement increases LH-RH-like mRNA level in the hypothalami of castrated rats suggesting that T may act at the pretranslational level. This change in LH-RH gene expression is parallel with alteration of LH-RH content and release.lld:pubmed
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pubmed-article:3075143pubmed:authorpubmed-author:KimKKlld:pubmed
pubmed-article:3075143pubmed:authorpubmed-author:ParkS DSDlld:pubmed
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pubmed-article:3075143pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3075143pubmed:articleTitleTestosterone stimulates LH-RH-like mRNA level in the rat hypothalamus.lld:pubmed
pubmed-article:3075143pubmed:affiliationDepartment of Zoology, College of Natural Sciences, Seoul National University, South Korea.lld:pubmed
pubmed-article:3075143pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3075143pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed