| pubmed-article:3040532 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C0999953 | lld:lifeskim |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:3040532 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:3040532 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3040532 | pubmed:dateCreated | 1987-10-15 | lld:pubmed |
| pubmed-article:3040532 | pubmed:abstractText | Sequences from genomic RNA segment 8 of the United Kingdom tissue-culture (t.c.)-adapted bovine rotavirus encoding a major viral neutralisation antigen VP7c have been expressed in Escherichia coli. Expression under the regulated control of the bacteriophage lambda pR promoter was as a C-terminal extension to E. coli beta-galactosidase (beta Gal). Following temperature induction, high levels of the fusion protein were synthesised and accumulated in induced cells, making up 5%-15% of total bacterial cell protein after 2 h of induction. Immunisation of sero-negative rabbits and mice with gel-purified fusion-protein raised antibodies, which gave specific immunofluorescence with virus-infected cells and were able to immunoprecipitate proteins of the VP7 complex from such cells. Hyperimmune sera also gave a virus-type-specific reaction in a solid-phase enzyme-linked immunoabsorbant assay and neutralised virus infectivity in standard plaque-reduction assays. | lld:pubmed |
| pubmed-article:3040532 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3040532 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3040532 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3040532 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3040532 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3040532 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3040532 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3040532 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:3040532 | pubmed:author | pubmed-author:McCraeM AMA | lld:pubmed |
| pubmed-article:3040532 | pubmed:author | pubmed-author:McCorquodaleJ... | lld:pubmed |
| pubmed-article:3040532 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3040532 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:3040532 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3040532 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3040532 | pubmed:pagination | 9-18 | lld:pubmed |
| pubmed-article:3040532 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:3040532 | pubmed:meshHeading | pubmed-meshheading:3040532-... | lld:pubmed |
| pubmed-article:3040532 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3040532 | pubmed:articleTitle | Expression of a major bovine rotavirus neutralisation antigen (VP7c) in Escherichia coli. | lld:pubmed |
| pubmed-article:3040532 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3040532 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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