| pubmed-article:3033896 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C0014665 | lld:lifeskim |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C0205253 | lld:lifeskim |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:3033896 | lifeskim:mentions | umls-concept:C0205087 | lld:lifeskim |
| pubmed-article:3033896 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3033896 | pubmed:dateCreated | 1987-5-26 | lld:pubmed |
| pubmed-article:3033896 | pubmed:abstractText | The regulation of equine herpesvirus type 1 (EHV-1) transcription was examined in infected rabbit kidney cells using metabolic inhibitors. In order to map EHV-1 immediate early, early, and late transcripts, viral RNA was 32P-labeled in vivo and hybridized to EHV-1 DNA restriction fragments immobilized on nitrocellulose filters. Immediate early viral RNA was mapped to one region of the viral genome within the inverted repeat DNA sequences (map units 0.78-0.83 and 0.95-1.0). Northern blot hybridization analysis using a 32P-labeled cloned DNA probe from this region identified a single immediate early viral transcript (approximately 6 kb). Transcription of early and late genes was not restricted to any specific region on the viral genome as indicated by the ability of 32P-labeled early and late RNA to hybridize to EHV-1 restriction endonuclease fragments from both the long and short components of EHV-1 DNA. Additional experiments performed without the use of metabolic inhibitors confirmed that EHV-1 transcription is temporally regulated. The characterization of EHV-1 transcription during productive infection will serve as a reference for the analysis of viral transcripts in oncogenically transformed and persistently infected cells. | lld:pubmed |
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| pubmed-article:3033896 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3033896 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3033896 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3033896 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3033896 | pubmed:month | May | lld:pubmed |
| pubmed-article:3033896 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:3033896 | pubmed:author | pubmed-author:GreyW HWH | lld:pubmed |
| pubmed-article:3033896 | pubmed:author | pubmed-author:O'CallaghanD... | lld:pubmed |
| pubmed-article:3033896 | pubmed:author | pubmed-author:CaughmanG BGB | lld:pubmed |
| pubmed-article:3033896 | pubmed:author | pubmed-author:BaumannR PRP | lld:pubmed |
| pubmed-article:3033896 | pubmed:author | pubmed-author:StaczekJJ | lld:pubmed |
| pubmed-article:3033896 | pubmed:author | pubmed-author:RobertsonA... | lld:pubmed |
| pubmed-article:3033896 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3033896 | pubmed:volume | 158 | lld:pubmed |
| pubmed-article:3033896 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3033896 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3033896 | pubmed:pagination | 79-87 | lld:pubmed |
| pubmed-article:3033896 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3033896 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3033896 | pubmed:articleTitle | Regulation of equine herpesvirus type 1 gene expression: characterization of immediate early, early, and late transcription. | lld:pubmed |
| pubmed-article:3033896 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3033896 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3033896 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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