| pubmed-article:3016725 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C0015161 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C1336767 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C1264633 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:3016725 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:3016725 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:3016725 | pubmed:dateCreated | 1986-9-25 | lld:pubmed |
| pubmed-article:3016725 | pubmed:abstractText | Detergent-soluble DNA is the fraction (2-4%) of DNA that is released into the supernate upon mild detergent lysis. It is nonmitochondrial in origin. It labels efficiently with deoxy[3H]ribonucleosides and the labeling is prevented by inhibitors of polymerase alpha and ribonucleotide reductase. In previous publications we have characterized detergent-soluble DNA from splenocytes of immunologically activated mice. In this publication we show that incorporation of [3H]thymidine into detergent-soluble DNA is prevented by pretreatment with novobiocin, 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA), and teniposide (VM26), three inhibitors of type II topoisomerases. Camptothecin, an inhibitor of type I topoisomerases, also reduces incorporation of [3H]thymidine but only to 50% of control levels. In addition to affecting incorporation of [3H]thymidine, preincubation with the topoisomerase II inhibitors m-AMSA and VM26 alters the amount of DNA recovered in the detergent-soluble fraction. At low concentrations of m-AMSA the amount of detergent-soluble DNA increases somewhat, whereas at higher drug concentrations a marked decrease is observed. Treatment with VM26 results in diminished amounts of DNA being released into the detergent-soluble fraction as well. However, maximal inhibition of detergent-soluble DNA release by VM26 requires the presence of camptothecin. Therefore, we suggest that topoisomerases play an important role in making a small part of lymphocyte chromatin detergent labile. Furthermore, these results are consistent with recent studies demonstrating a role for topoisomerases in yeast replication. Thus, the newly synthesized portion of detergent-soluble DNA may arise as DNA replication intermediates not yet stabilized into mature chromatin. | lld:pubmed |
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| pubmed-article:3016725 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3016725 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3016725 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3016725 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3016725 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3016725 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3016725 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3016725 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:3016725 | pubmed:author | pubmed-author:StraussP RPR | lld:pubmed |
| pubmed-article:3016725 | pubmed:author | pubmed-author:ZhangL HLH | lld:pubmed |
| pubmed-article:3016725 | pubmed:author | pubmed-author:TodtJ CJC | lld:pubmed |
| pubmed-article:3016725 | pubmed:author | pubmed-author:SunY BYB | lld:pubmed |
| pubmed-article:3016725 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3016725 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:3016725 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3016725 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3016725 | pubmed:pagination | 5871-4 | lld:pubmed |
| pubmed-article:3016725 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3016725 | pubmed:meshHeading | pubmed-meshheading:3016725-... | lld:pubmed |
| pubmed-article:3016725 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3016725 | pubmed:articleTitle | Role for topoisomerases in the release of DNA into the detergent-soluble fraction of eukaryotic cells. | lld:pubmed |
| pubmed-article:3016725 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3016725 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3016725 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
