| pubmed-article:3002274 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0085236 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0017817 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0027303 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0076150 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:3002274 | lifeskim:mentions | umls-concept:C0085416 | lld:lifeskim |
| pubmed-article:3002274 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3002274 | pubmed:dateCreated | 1986-1-30 | lld:pubmed |
| pubmed-article:3002274 | pubmed:abstractText | The effects of t-butyl hydroperoxide on glutathione and NADPH and the respiratory burst (an NADPH-dependent function) in rat alveolar macrophages was investigated. Alveolar macrophages were exposed for 15 min to t-butyl hydroperoxide in the presence or absence of added glucose. Cells were then assayed for concanavalin A-stimulated O2 production or for NADPH, NADP, reduced glutathione, glutathione disulfide, glutathione released into the medium and glutathione mixed disulfides. Exposure of rat alveolar macrophages to 1 X 10(-5) M t-butyl hydroperoxide causes a loss of concanavalin A-stimulated superoxide production (the respiratory burst) that can be prevented or reversed by added glucose. Cells incubated without glucose had a higher oxidation state of the NADPH/NADP couple than cells incubated with glucose. With t-butyl hydroperoxide, NADP rose to almost 100% of the NADP + NADPH pool; however, addition of glucose prevented this alteration of the NADPH oxidation state. Cells exposed to 1 X 10(-5) M t-butyl hydroperoxide in the absence of glucose showed a significant increase in the percentage GSSG in the GSH + GSSG pool and increased glutathione mixed disulfides. These changes in glutathione distribution could also be prevented or reversed by glucose. With 1 X 10(-4) M t-butyl hydroperoxide, changes in glutathione oxidation were not prevented by glucose and cells were irreversibly damaged. We conclude that drastic alteration of the NADPH/NADP ratio does not itself reflect toxicity and that significant alteration of glutathione distribution can also be tolerated; however, when oxidative stress exceeds the ability of glucose to prevent alterations in oxidation state, irreversible damage to cell function and structure may occur. | lld:pubmed |
| pubmed-article:3002274 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3002274 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3002274 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3002274 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3002274 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:3002274 | pubmed:issn | 0003-9861 | lld:pubmed |
| pubmed-article:3002274 | pubmed:author | pubmed-author:NelsonJJ | lld:pubmed |
| pubmed-article:3002274 | pubmed:author | pubmed-author:FormanH JHJ | lld:pubmed |
| pubmed-article:3002274 | pubmed:author | pubmed-author:HarrisonGG | lld:pubmed |
| pubmed-article:3002274 | pubmed:author | pubmed-author:SutherlandM... | lld:pubmed |
| pubmed-article:3002274 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3002274 | pubmed:volume | 243 | lld:pubmed |
| pubmed-article:3002274 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3002274 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3002274 | pubmed:pagination | 325-31 | lld:pubmed |
| pubmed-article:3002274 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
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| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:meshHeading | pubmed-meshheading:3002274-... | lld:pubmed |
| pubmed-article:3002274 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3002274 | pubmed:articleTitle | Effects of t-butyl hydroperoxide on NADPH, glutathione, and the respiratory burst of rat alveolar macrophages. | lld:pubmed |
| pubmed-article:3002274 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3002274 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:3002274 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3002274 | lld:pubmed |
