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pubmed-article:2983879pubmed:abstractTextA human hepatoma cell line (HepG-2) was probed for the presence of lecithin: cholesterol acyltransferase (LCAT) using an antiserum to human plasma LCAT. Double immunodiffusion analysis using antiserum to human plasma LCAT revealed a single precipitin line in the sonicated cell homogenate. This precipitin line showed a reaction of identity with highly purified plasma LCAT. The presence of LCAT within the hepatoma cells was also confirmed by an immunofluorescence test. In contrast, the cell culture supernate showed a weak and inconsistent precipitin line. These data suggest that HepG-2 cells synthesize LCAT but secretion of the enzyme by these cells into the culture medium may be partially or totally impaired.lld:pubmed
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pubmed-article:2983879pubmed:articleTitleDetection of lecithin: cholesterol acyltransferase (LCAT) in a human hepatoma cell line.lld:pubmed
pubmed-article:2983879pubmed:publicationTypeJournal Articlelld:pubmed
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