| pubmed-article:2982850 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2982850 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:2982850 | lifeskim:mentions | umls-concept:C0013729 | lld:lifeskim |
| pubmed-article:2982850 | lifeskim:mentions | umls-concept:C1158884 | lld:lifeskim |
| pubmed-article:2982850 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:2982850 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:2982850 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2982850 | pubmed:dateCreated | 1985-4-11 | lld:pubmed |
| pubmed-article:2982850 | pubmed:abstractText | Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2) causes mRNA to accumulate in 48 S complexes containing Met-tRNAf and eIF-2(alpha P). When the eIF-2 alpha kinase is inhibited by 2-aminopurine, the mRNA is slowly transferred from 48 to 80 S initiation complexes after an initial lag. The cause of this lag was examined by investigating whether mRNA and Met-tRNAf dissociated from 48 S complexes before binding to 80 S. Both compounds were quantitatively transferred from 48 to 80 S complexes after addition of 2-aminopurine and the eIF-2(alpha P) bound to 48 S complexes was dephosphorylated after an initial lag more slowly than unbound eIF-2(alpha P), which was rapidly dephosphorylated. the eIF-2(alpha P) in isolated 48 S complexes was slowly dephosphorylated by partially purified lysate phosphatases, whereas free eIF-2(alpha P) was readily dephosphorylated. These results indicated that 48 S complexes could directly join to a 60 S ribosomal subunit after eIF-2(alpha P) dephosphorylation. The lag and slow kinetics of dephosphorylation of eIF-2(alpha P) bound to 48 S complexes accounted for the slow transfer of mRNA from 48 to 80 S complexes. Moreover, the mRNA bound to 48 S complexes was more susceptible to cleavage by an endonuclease than mRNA in polyribosomes, as shown by activating the (2'-5')oligo(A)-dependent endonuclease. This finding is discussed in view of the possible role of eIF-2 alpha kinase and endonuclease in the inhibition of viral mRNA translation in interferon-treated cells. | lld:pubmed |
| pubmed-article:2982850 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2982850 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2982850 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2982850 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2982850 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2982850 | pubmed:author | pubmed-author:BaglioniCC | lld:pubmed |
| pubmed-article:2982850 | pubmed:author | pubmed-author:De... | lld:pubmed |
| pubmed-article:2982850 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2982850 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:2982850 | pubmed:volume | 260 | lld:pubmed |
| pubmed-article:2982850 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2982850 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2982850 | pubmed:pagination | 3135-9 | lld:pubmed |
| pubmed-article:2982850 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
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| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
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| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:meshHeading | pubmed-meshheading:2982850-... | lld:pubmed |
| pubmed-article:2982850 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2982850 | pubmed:articleTitle | Kinetics of dephosphorylation of eIF-2(alpha P) and reutilization of mRNA. | lld:pubmed |
| pubmed-article:2982850 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2982850 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2982850 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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