Linked Life Data

2918843

Source:http://linkedlifedata.com/resource/pubmed/id/2918843

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pubmed-article:2918843pubmed:abstractTextThe roles of subcellular components and, in particular, cytosol fractions and beta-nicotinamide adenine dinucleotide phosphate, reduced form (NADPH), in the regulation of rat hepatic and renal 5'-deiodination during fasting were assessed. 5'-deiodinase (5'-DI) activities in reaction mixtures were measured by using outer ring 125I-radiolabelled reverse triiodothyronine (rT3) as a substrate in the presence of 200 mumol/L NADPH. Subcellular components from rats fed ad libitum or fasted for 24, 48 or 72 hours were prepared by standard differential centrifugation. Cytosol was chromatographed on a Sephadex G-50 column to obtain Fraction A of molecular weight (Mr) greater than 60,000 and Fraction B of Mr approximately 13,000 and to exclude reduced glutathione (GSH) (Mr less than 400). 5'-DI activity in liver homogenates was reduced by 42% at 24 hours and by 59% at 48 hours of fasting. In reconstitution experiments, liver microsomes showed a progressive loss of 5'-DI activity, reaching a maximal reduction of 46% at 72 hours of fasting. Activation of microsomal deiodinase by whole liver cytosol was also significantly reduced at 24 hours of fasting and achieved a maximal reduction of 5'-DI activation of 42% at 48 hours before substantial but incomplete recovery at 72 hours. Cytosolic Fraction A and B were assessed in combination with fed microsomes and NADPH. A close correlation was demonstrated between the loss of hepatic 5'-DI supportive activity in whole cytosol and that of Fraction B but not A during fasting.(ABSTRACT TRUNCATED AT 250 WORDS)lld:pubmed
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pubmed-article:2918843pubmed:pagination230-7lld:pubmed
pubmed-article:2918843pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:2918843pubmed:year1989lld:pubmed
pubmed-article:2918843pubmed:articleTitleRat hepatic and renal 5'-deiodination of rT3 during fasting: supportive role of intermediate Mr cytosolic non-glutathione thiol cofactor and NADPH.lld:pubmed
pubmed-article:2918843pubmed:affiliationThyroid Research Laboratory, Mount Sinai Hospital, Toronto, Ontario, Canada.lld:pubmed
pubmed-article:2918843pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2918843pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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