| pubmed-article:2918036 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2918036 | lifeskim:mentions | umls-concept:C0031715 | lld:lifeskim |
| pubmed-article:2918036 | lifeskim:mentions | umls-concept:C0282549 | lld:lifeskim |
| pubmed-article:2918036 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2918036 | lifeskim:mentions | umls-concept:C0040845 | lld:lifeskim |
| pubmed-article:2918036 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:2918036 | lifeskim:mentions | umls-concept:C0205227 | lld:lifeskim |
| pubmed-article:2918036 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2918036 | pubmed:dateCreated | 1989-3-29 | lld:pubmed |
| pubmed-article:2918036 | pubmed:abstractText | HL-60 cells were incubated with [32P]-Pi in order to label endogenous phosphoproteins in situ. These were then resolved via two-dimensional electrophoresis and autoradiograms were made from the gels. A comparison of autoradiograms made from retinoic-acid-differentiated cells with those made from control cells revealed a small number of phosphoproteins whose labeling was enhanced in differentiated cells. Incubating HL-60 cells with [35S]-methionine revealed that RA induced the synthesis of one of these proteins, (53 kDa, pl 4.8) although not to a degree sufficient to account for the increased phosphate labeling observed in differentiated cells. Difluoromethylornithine (DFMO), which arrests HL-60 cell proliferation without inducing differentiation, had no effect on endogenous protein phosphorylation. Treatment of DFMO-arrested cells with retinoic acid, however, increased the phosphorylation of the proteins and resulted in differentiation of the cells. Densitometric analysis of autoradiograms made from two-dimensional gels revealed that the phosphorylation of the 53-kDa, pl 4.8 protein was significantly elevated in cells exposed to RA for as little as 12 hours, i.e., before the cells were committed to differentiate and before a significant increase in the number of phenotypically mature cells was observed. It therefore appears that this protein may be an intermediate in the retinoic-acid-induced differentiation process. | lld:pubmed |
| pubmed-article:2918036 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2918036 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2918036 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2918036 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2918036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2918036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2918036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2918036 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2918036 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2918036 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:2918036 | pubmed:author | pubmed-author:DurhamJ PJP | lld:pubmed |
| pubmed-article:2918036 | pubmed:author | pubmed-author:ButcherF RFR | lld:pubmed |
| pubmed-article:2918036 | pubmed:author | pubmed-author:SpearmanT NTN | lld:pubmed |
| pubmed-article:2918036 | pubmed:author | pubmed-author:FontanaJ AJA | lld:pubmed |
| pubmed-article:2918036 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2918036 | pubmed:volume | 138 | lld:pubmed |
| pubmed-article:2918036 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2918036 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2918036 | pubmed:pagination | 349-57 | lld:pubmed |
| pubmed-article:2918036 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:meshHeading | pubmed-meshheading:2918036-... | lld:pubmed |
| pubmed-article:2918036 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2918036 | pubmed:articleTitle | Effect of retinoic acid on the phosphorylation of endogenous proteins in HL-60 cells. | lld:pubmed |
| pubmed-article:2918036 | pubmed:affiliation | Department of Biochemistry, West Virginia University School of Medicine, Morgantown 26506. | lld:pubmed |
| pubmed-article:2918036 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2918036 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2918036 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2918036 | lld:pubmed |
