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pubmed-article:2891611pubmed:abstractTextWe have isolated an unusual T cell receptor gamma chain cDNA clone (gamma 7.1) from a library made from RNA derived from adult thymus of C57BL/Ka mice. This cDNA clone corresponds to the appropriately processed C gamma 1 constant region exons preceded by 1.5 kb of J-C gamma 1 intron. The gamma 7.1 coding region is extremely homologous to the C gamma 1 gene of BALB/c mice, differing at the protein level by a single deletion (alanine 139) and a single substitution. This latter change eliminates the sole N-linked sugar attachment site, providing a basis for strain-specific glycosylation patterns. The J-C gamma 1 intronic region contains two DNA segments (termed psi J gamma 1 and psi J gamma 2) that are highly reminiscent of joining (J) segments; both have potentially functional recombination and donor splice sequences flanking an open reading frame. Northern analysis suggests that gamma 7.1 may be derived from a large, variable region-containing precursor.lld:pubmed
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pubmed-article:2891611pubmed:pagination121-6lld:pubmed
pubmed-article:2891611pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2891611pubmed:articleTitleDiversity of the mouse T cell receptor C gamma 1 gene: structural analysis in C57BL/Ka.lld:pubmed
pubmed-article:2891611pubmed:affiliationDepartment of Microbiology, University of Texas Health Science Center, Dallas 75235.lld:pubmed
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pubmed-article:2891611pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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