| pubmed-article:2890388 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2890388 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:2890388 | lifeskim:mentions | umls-concept:C0005955 | lld:lifeskim |
| pubmed-article:2890388 | lifeskim:mentions | umls-concept:C0014819 | lld:lifeskim |
| pubmed-article:2890388 | lifeskim:mentions | umls-concept:C1828121 | lld:lifeskim |
| pubmed-article:2890388 | lifeskim:mentions | umls-concept:C0443252 | lld:lifeskim |
| pubmed-article:2890388 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2890388 | pubmed:dateCreated | 1988-1-21 | lld:pubmed |
| pubmed-article:2890388 | pubmed:abstractText | The spleen colony-forming assay does not represent the number of hematopoietic stem cells with extensive self-maintaining capacity because five to 50 spleen colony-forming units (CFU-S) are necessary to rescue a genetically anemic (WB X C57BL/6)F1-W/Wv(WBB6F1-W/Wv) mouse. We investigated which is more important for the reconstitution of erythropoiesis, the transplantation of multiple CFU-S or that of a single stem cell with extensive self-maintaining potential. The electrophoretic pattern of hemoglobin was used as a marker of reconstitution and that of phosphoglycerate kinase (PGK), an X chromosome-linked enzyme, as a tool for estimating the number of stem cells. For this purpose, we developed the C57BL/6 congeneic strain with the Pgk-1a gene. Bone marrow cells were harvested after injection of 5-fluorouracil from C57BL/6-Pgk-1b/Pgk-1a female mice in which each stem cell had either A-type PGK or B-type PGK due to the random inactivation of one or two X chromosomes. When a relatively small number of bone marrow cells (ie, 10(3) or 3 X 10(3] were injected into 200-rad-irradiated WBB6F1-W/Wv mice, the hemoglobin pattern changed from the recipient type (Hbbd/Hbbs) to the donor type (Hbbs/Hbbs) in seven of 150 mice for at least 8 weeks. Erythrocytes of all these WBB6F1-W/Wv mice showed either A-type PGK alone or B-type PGK alone during the time of reconstitution, which suggests that a single stem cell with extensive self-maintaining potential may sustain the whole erythropoiesis of a mouse for at least 8 weeks. | lld:pubmed |
| pubmed-article:2890388 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2890388 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2890388 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2890388 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2890388 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2890388 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2890388 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2890388 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:2890388 | pubmed:author | pubmed-author:AsaiHH | lld:pubmed |
| pubmed-article:2890388 | pubmed:author | pubmed-author:NakanoTT | lld:pubmed |
| pubmed-article:2890388 | pubmed:author | pubmed-author:KitamuraYY | lld:pubmed |
| pubmed-article:2890388 | pubmed:author | pubmed-author:WakoHH | lld:pubmed |
| pubmed-article:2890388 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2890388 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:2890388 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2890388 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2890388 | pubmed:pagination | 1758-63 | lld:pubmed |
| pubmed-article:2890388 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:meshHeading | pubmed-meshheading:2890388-... | lld:pubmed |
| pubmed-article:2890388 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2890388 | pubmed:articleTitle | Long-term monoclonal reconstitution of erythropoiesis in genetically anemic W/Wv mice by injection of 5-fluorouracil-treated bone marrow cells of Pgk-1b/Pgk-1a mice. | lld:pubmed |
| pubmed-article:2890388 | pubmed:affiliation | Division of Cancer Pathology, Osaka University Medical School, Japan. | lld:pubmed |
| pubmed-article:2890388 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2890388 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2890388 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2890388 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2890388 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2890388 | lld:pubmed |
