| pubmed-article:2743404 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C0018787 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C0445223 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C1552599 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C1704787 | lld:lifeskim |
| pubmed-article:2743404 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
| pubmed-article:2743404 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2743404 | pubmed:dateCreated | 1989-8-17 | lld:pubmed |
| pubmed-article:2743404 | pubmed:abstractText | A simple procedure is described for the purification of the alpha alpha isoform of S-100 proteins (S-100a0) from porcine heart. Purification steps include the following: i) extraction of the tissue with a hypotonic medium containing EDTA; ii) ammonium sulfate fractionation (0-50%) of the extract; iii) Ca2+-dependent affinity chromatography of the supernatant obtained through the preceding step on phenyl-sepharose and elution of absorbed proteins through a two-chamber gradient of 1.0-0.0 mM CaCl2 and 0.0--1.0 mM EGTA, respectively; and iv) chromatography of the resultant S-100-containing fractions on Sephadex G-200. The yield is 20 mg S-100a0/kg porcine heart. The whole procedure takes five days and is highly reproducible. Data obtained from the phenyl-sepharose step suggest that the affinity of Ca2+ for S-100a0 increases by several orders of magnitude once the protein had interacted with that matrix. This observation is discussed in relation to the role of S-100 proteins in amplification of the Ca2+ signal. Immunocytochemical and immunoblotting analyses indicate that S-100a0 is exclusively found at the level of the sarcolemmal membranes, the membranes of the sarcoplasmic reticulum, the external mitochondrial membranes, and in the adjacent sarcoplasm. No evidence of S-100a0 being associated with the nuclei or with myofibrils has been obtained. Finally, the cardiac tissue does not contain the Triton X-100-extractable fraction of S-100 normally detected in the brain and in adipocytes. Our data suggest that S-100a0 behaves as a peripheral membrane protein in cardiac tissue. | lld:pubmed |
| pubmed-article:2743404 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2743404 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2743404 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2743404 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2743404 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2743404 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2743404 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2743404 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2743404 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2743404 | pubmed:issn | 0143-4160 | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:DonatoRR | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:CeccarelliPP | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:SprecaAA | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:RambottiM GMG | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:GiambancoII | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:AisaM CMC | lld:pubmed |
| pubmed-article:2743404 | pubmed:author | pubmed-author:di GeronimoGG | lld:pubmed |
| pubmed-article:2743404 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2743404 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:2743404 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2743404 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2743404 | pubmed:pagination | 81-92 | lld:pubmed |
| pubmed-article:2743404 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
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| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:meshHeading | pubmed-meshheading:2743404-... | lld:pubmed |
| pubmed-article:2743404 | pubmed:articleTitle | Cardiac S-100a0 protein: purification by a simple procedure and related immunocytochemical and immunochemical studies. | lld:pubmed |
| pubmed-article:2743404 | pubmed:affiliation | Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. | lld:pubmed |
| pubmed-article:2743404 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2743404 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
