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pubmed-article:2695530pubmed:abstractTextThe beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubulin antigen in the mitotic spindle of the mature plasmodium - the sole microtubular array in that stage of Physarum. By immunofluorescence, the beta 2-tubulin antigen was detected throughout this anastral mitotic spindle, at all stages of mitosis. Physarum myxamoebae contain astral mitotic spindles and cytoskeletal microtubules. No beta 2-tubulin antigen was detected in the myxamoebal stage. However, as cultures of myxamoebae developed into plasmodia, the beta 2-tubulin antigen was found in the astral mitotic spindles and cytoskeletons in developing cells. Thus, the presence of the plasmodial beta 2-tubulin isotype in a mitotic spindle does not determine a closed, anastral mitosis.lld:pubmed
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pubmed-article:2695530pubmed:monthOctlld:pubmed
pubmed-article:2695530pubmed:issn0021-9533lld:pubmed
pubmed-article:2695530pubmed:authorpubmed-author:DoveW FWFlld:pubmed
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pubmed-article:2695530pubmed:authorpubmed-author:BurlandT GTGlld:pubmed
pubmed-article:2695530pubmed:authorpubmed-author:PaulE CEClld:pubmed
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pubmed-article:2695530pubmed:issnTypePrintlld:pubmed
pubmed-article:2695530pubmed:volume94 ( Pt 2)lld:pubmed
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pubmed-article:2695530pubmed:pagination217-26lld:pubmed
pubmed-article:2695530pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2695530pubmed:year1989lld:pubmed
pubmed-article:2695530pubmed:articleTitleThe localization of the divergent beta 2-tubulin isotype in the microtubular arrays of Physarum polycephalum.lld:pubmed
pubmed-article:2695530pubmed:affiliationMcArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.lld:pubmed
pubmed-article:2695530pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2695530pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2695530pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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