| pubmed-article:2679911 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C0162597 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C0376152 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C2753498 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2679911 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2679911 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2679911 | pubmed:dateCreated | 1989-12-8 | lld:pubmed |
| pubmed-article:2679911 | pubmed:abstractText | Production of interleukin-6 (IL-6) by marrow stromal cells from human long-term marrow cultures and from stromal cells transformed with simian virus 40 was examined. As with other cultured mesenchymal cells, unstimulated stromal cells produced undetectable amounts of IL-6 mRNA when assayed by Northern blots. However, within 30 minutes after exposure of transformed marrow stromal cells to the inflammatory mediators, recombinant human interleukin-1 alpha (IL-1 alpha) or recombinant human tumor necrosis factor alpha (TNF alpha), significant increases in IL-6 expression were observed. The time course of IL-6 mRNA upregulation in transformed marrow stromal cells with IL-1 alpha and TNF alpha differed: The maximal response to TNF alpha was observed at 30 minutes whereas that to IL-1 alpha occurred at 8 hours. Although IL-6 at a concentration of 500 U/mL was inhibitory to adherent transformed marrow stromal cell proliferation, a concentration-dependent stimulation of anchorage-independent colony growth was observed when the cells were plated in semisolid medium with IL-6. The stromal cell colony-stimulating effect of IL-6 was abrogated by a neutralizing antibody to IL-6. Moreover, the heteroserum with anti-IL-6 activity and two anti-IL-6 monoclonal antibodies partially blocked autonomous and IL-1 alpha-induced colony formation, suggesting that colony formation by transformed marrow stromal cells may require IL-6. Clonal-transformed stromal cell lines were derived from the anchorage-independent stromal cell colonies. Both IL-6 mRNA and protein were constitutively produced at high levels. The addition of IL-6 to either long-term marrow culture adherent cells or transformed marrow stromal cells downregulated the expression of collagen I, a major stromal cell matrix protein. Thus, IL-6 affects proliferation of stromal cells and influences their production of extracellular matrix, suggesting that IL-6 may have indirect as well as direct influences on hematopoietic cell proliferation. | lld:pubmed |
| pubmed-article:2679911 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2679911 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2679911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2679911 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2679911 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:2679911 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:2679911 | pubmed:author | pubmed-author:SingerJ WJW | lld:pubmed |
| pubmed-article:2679911 | pubmed:author | pubmed-author:LillyM BMB | lld:pubmed |
| pubmed-article:2679911 | pubmed:author | pubmed-author:NemunaitisJJ | lld:pubmed |
| pubmed-article:2679911 | pubmed:author | pubmed-author:AndrewsD FDF | lld:pubmed |
| pubmed-article:2679911 | pubmed:author | pubmed-author:MochizukiD... | lld:pubmed |
| pubmed-article:2679911 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2679911 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2679911 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:2679911 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2679911 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2679911 | pubmed:pagination | 1929-35 | lld:pubmed |
| pubmed-article:2679911 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
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| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:meshHeading | pubmed-meshheading:2679911-... | lld:pubmed |
| pubmed-article:2679911 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2679911 | pubmed:articleTitle | Human marrow stromal cells: response to interleukin-6 (IL-6) and control of IL-6 expression. | lld:pubmed |
| pubmed-article:2679911 | pubmed:affiliation | Medical Service, Veterans Administration Medical Center, Seattle, WA 98108. | lld:pubmed |
| pubmed-article:2679911 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2679911 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2679911 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2679911 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2679911 | lld:pubmed |
