| pubmed-article:2675490 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0315120 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0017982 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:2675490 | lifeskim:mentions | umls-concept:C0014154 | lld:lifeskim |
| pubmed-article:2675490 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2675490 | pubmed:dateCreated | 1989-10-20 | lld:pubmed |
| pubmed-article:2675490 | pubmed:abstractText | The Clostridium thermocellum celA gene encoding endoglucanase A is expressed in Saccharomyces cerevisiae but the enzyme produced from the native celA gene is not secreted. After removal of the bacterial signal peptide-coding sequence, the gene was fused to the promoter and prepro segment of the S. cerevisiae MF alpha 1 gene. This construction directs secretion of active endoglucanase A into the culture medium when introduced in yeast on either replicating or integrating vectors. Secretion of endoglucanase A required growth of transformants on rich medium. The secreted enzyme is a 97,000 Da glycoprotein containing about half of its molecular weight as carbohydrate. This new gene fusion could facilitate further research on protein secretion in yeast by using a cellulase as a marker enzyme. | lld:pubmed |
| pubmed-article:2675490 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2675490 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2675490 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2675490 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2675490 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2675490 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2675490 | pubmed:issn | 0749-503X | lld:pubmed |
| pubmed-article:2675490 | pubmed:author | pubmed-author:SilvaAA | lld:pubmed |
| pubmed-article:2675490 | pubmed:author | pubmed-author:VazquezRR | lld:pubmed |
| pubmed-article:2675490 | pubmed:author | pubmed-author:BenitezJJ | lld:pubmed |
| pubmed-article:2675490 | pubmed:author | pubmed-author:HollenbergC... | lld:pubmed |
| pubmed-article:2675490 | pubmed:author | pubmed-author:NoaM DMD | lld:pubmed |
| pubmed-article:2675490 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2675490 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:2675490 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2675490 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2675490 | pubmed:pagination | 299-306 | lld:pubmed |
| pubmed-article:2675490 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:meshHeading | pubmed-meshheading:2675490-... | lld:pubmed |
| pubmed-article:2675490 | pubmed:articleTitle | Secretion and glycosylation of Clostridium thermocellum endoglucanase A encoded by the celA gene in Saccharomyces cerevisiae. | lld:pubmed |
| pubmed-article:2675490 | pubmed:affiliation | Departamento de Genética, Centro Nacional de Investigaciones Cientificas, La Habana, Cuba. | lld:pubmed |
| pubmed-article:2675490 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2675490 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
